Enhancing the Heterologous Expression of a Thermophilic Endoglucanase and Its Cost-Effective Production in Pichia pastoris Using Multiple Strategies

Although Pichia pastoris was successfully used for heterologous gene expression for more than twenty years, many factors influencing protein expression remain unclear. Here, we optimized the expression of a thermophilic endoglucanase from Thermothielavioides terrestris (TtCel45A) for cost-effective...

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Detalles Bibliográficos
Autores principales: Dai, Wuling, Dong, Haofan, Zhang, Zhaokun, Wu, Xin, Bao, Tongtong, Gao, Le, Chen, Xiaoyi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10573353/
https://www.ncbi.nlm.nih.gov/pubmed/37834464
http://dx.doi.org/10.3390/ijms241915017

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10573353/
https://www.ncbi.nlm.nih.gov/pubmed/37834464
http://dx.doi.org/10.3390/ijms241915017

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