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Genome and Transcriptome Analyses of Genes Involved in Ascorbate Biosynthesis in Pepper Indicate Key Genes Related to Fruit Development, Stresses, and Phytohormone Exposures

Pepper (Capsicum annuum L.) is a vegetable consumed worldwide, primarily used for vitamin C uptake and condiment purposes. Ascorbate (Asc) is a multifunctional metabolite, acting as an antioxidant and enzymatic cofactor involved in multiple cellular processes. Nevertheless, there is no evidence abou...

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Autores principales: de Aguiar, Évelyn Silva, Dias, Abigailde Nascimento, Sousa, Raquel Mendes, Germano, Thais Andrade, de Sousa, Renato Oliveira, Miranda, Rafael de Souza, Costa, José Hélio, dos Santos, Clesivan Pereira
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10574469/
https://www.ncbi.nlm.nih.gov/pubmed/37836106
http://dx.doi.org/10.3390/plants12193367
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author de Aguiar, Évelyn Silva
Dias, Abigailde Nascimento
Sousa, Raquel Mendes
Germano, Thais Andrade
de Sousa, Renato Oliveira
Miranda, Rafael de Souza
Costa, José Hélio
dos Santos, Clesivan Pereira
author_facet de Aguiar, Évelyn Silva
Dias, Abigailde Nascimento
Sousa, Raquel Mendes
Germano, Thais Andrade
de Sousa, Renato Oliveira
Miranda, Rafael de Souza
Costa, José Hélio
dos Santos, Clesivan Pereira
author_sort de Aguiar, Évelyn Silva
collection PubMed
description Pepper (Capsicum annuum L.) is a vegetable consumed worldwide, primarily used for vitamin C uptake and condiment purposes. Ascorbate (Asc) is a multifunctional metabolite, acting as an antioxidant and enzymatic cofactor involved in multiple cellular processes. Nevertheless, there is no evidence about the contribution of biosynthesis pathways and regulatory mechanisms responsible for Asc reserves in pepper plants. Here, we present a genome- and transcriptome-wide investigation of genes responsible for Asc biosynthesis in pepper during fruit development, stresses, and phytohormone exposures. A total of 21 genes, scattered in ten of twelve pepper chromosomes were annotated. Gene expression analyses of nine transcriptomic experiments supported the primary role of the L-galactose pathway in the Asc-biosynthesizing process, given its constitutive, ubiquitous, and high expression profile observed in all studied conditions. However, genes from alternative pathways generally exhibited low expression or were unexpressed and appeared to play some secondary role under specific stress conditions and phytohormone treatments. Taken together, our findings provide a deeper spatio-temporal understanding of expression levels of genes involved in Asc biosynthesis, and they highlight GGP2, GME1 and 2, and GalLDH members from L-galactose pathway as promising candidates for future wet experimentation, addressing the attainment of increase in ascorbate content of peppers and other crops.
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spelling pubmed-105744692023-10-14 Genome and Transcriptome Analyses of Genes Involved in Ascorbate Biosynthesis in Pepper Indicate Key Genes Related to Fruit Development, Stresses, and Phytohormone Exposures de Aguiar, Évelyn Silva Dias, Abigailde Nascimento Sousa, Raquel Mendes Germano, Thais Andrade de Sousa, Renato Oliveira Miranda, Rafael de Souza Costa, José Hélio dos Santos, Clesivan Pereira Plants (Basel) Article Pepper (Capsicum annuum L.) is a vegetable consumed worldwide, primarily used for vitamin C uptake and condiment purposes. Ascorbate (Asc) is a multifunctional metabolite, acting as an antioxidant and enzymatic cofactor involved in multiple cellular processes. Nevertheless, there is no evidence about the contribution of biosynthesis pathways and regulatory mechanisms responsible for Asc reserves in pepper plants. Here, we present a genome- and transcriptome-wide investigation of genes responsible for Asc biosynthesis in pepper during fruit development, stresses, and phytohormone exposures. A total of 21 genes, scattered in ten of twelve pepper chromosomes were annotated. Gene expression analyses of nine transcriptomic experiments supported the primary role of the L-galactose pathway in the Asc-biosynthesizing process, given its constitutive, ubiquitous, and high expression profile observed in all studied conditions. However, genes from alternative pathways generally exhibited low expression or were unexpressed and appeared to play some secondary role under specific stress conditions and phytohormone treatments. Taken together, our findings provide a deeper spatio-temporal understanding of expression levels of genes involved in Asc biosynthesis, and they highlight GGP2, GME1 and 2, and GalLDH members from L-galactose pathway as promising candidates for future wet experimentation, addressing the attainment of increase in ascorbate content of peppers and other crops. MDPI 2023-09-23 /pmc/articles/PMC10574469/ /pubmed/37836106 http://dx.doi.org/10.3390/plants12193367 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
de Aguiar, Évelyn Silva
Dias, Abigailde Nascimento
Sousa, Raquel Mendes
Germano, Thais Andrade
de Sousa, Renato Oliveira
Miranda, Rafael de Souza
Costa, José Hélio
dos Santos, Clesivan Pereira
Genome and Transcriptome Analyses of Genes Involved in Ascorbate Biosynthesis in Pepper Indicate Key Genes Related to Fruit Development, Stresses, and Phytohormone Exposures
title Genome and Transcriptome Analyses of Genes Involved in Ascorbate Biosynthesis in Pepper Indicate Key Genes Related to Fruit Development, Stresses, and Phytohormone Exposures
title_full Genome and Transcriptome Analyses of Genes Involved in Ascorbate Biosynthesis in Pepper Indicate Key Genes Related to Fruit Development, Stresses, and Phytohormone Exposures
title_fullStr Genome and Transcriptome Analyses of Genes Involved in Ascorbate Biosynthesis in Pepper Indicate Key Genes Related to Fruit Development, Stresses, and Phytohormone Exposures
title_full_unstemmed Genome and Transcriptome Analyses of Genes Involved in Ascorbate Biosynthesis in Pepper Indicate Key Genes Related to Fruit Development, Stresses, and Phytohormone Exposures
title_short Genome and Transcriptome Analyses of Genes Involved in Ascorbate Biosynthesis in Pepper Indicate Key Genes Related to Fruit Development, Stresses, and Phytohormone Exposures
title_sort genome and transcriptome analyses of genes involved in ascorbate biosynthesis in pepper indicate key genes related to fruit development, stresses, and phytohormone exposures
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10574469/
https://www.ncbi.nlm.nih.gov/pubmed/37836106
http://dx.doi.org/10.3390/plants12193367
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