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Optimized nucleus isolation protocol from frozen mouse tissues for single nucleus RNA sequencing application

The single cell RNA sequencing technique has been particularly used during the last years, allowing major discoveries. However, the widespread application of this analysis has showed limitations. Indeed, the direct study of fresh tissues is not always feasible, notably in the case of genetically eng...

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Detalles Bibliográficos
Autores principales: Minati, Marie-Albane, Fages, Angeline, Dauguet, Nicolas, Zhu, Jingjing, Jacquemin, Patrick
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10575574/
https://www.ncbi.nlm.nih.gov/pubmed/37842081
http://dx.doi.org/10.3389/fcell.2023.1243863