Cargando…

Characterization of Potential Probiotic Activity of Lactic Acid Bacteria Isolated from Camel Colostrum by Biochemical and Molecular Methods

A total of 60 isolates of lactic acid bacteria (LAB) were isolated from Jordanian camel colostrum using biochemical and molecular methods. Two dominant species were identified, and they were Lactobacillus salivarius and Enterococcus faecium. The entire 60 isolated LAB were tested for their acidity a...

Descripción completa

Detalles Bibliográficos
Autores principales: Safi, Enas, Haddad, Moawiya, Hasan, Maen, Al-Dalain, Sati Y., Proestos, Charalampos, Siddiqui, Shahida A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10576644/
https://www.ncbi.nlm.nih.gov/pubmed/37841508
http://dx.doi.org/10.1155/2023/8334152
_version_ 1785121159543521280
author Safi, Enas
Haddad, Moawiya
Hasan, Maen
Al-Dalain, Sati Y.
Proestos, Charalampos
Siddiqui, Shahida A.
author_facet Safi, Enas
Haddad, Moawiya
Hasan, Maen
Al-Dalain, Sati Y.
Proestos, Charalampos
Siddiqui, Shahida A.
author_sort Safi, Enas
collection PubMed
description A total of 60 isolates of lactic acid bacteria (LAB) were isolated from Jordanian camel colostrum using biochemical and molecular methods. Two dominant species were identified, and they were Lactobacillus salivarius and Enterococcus faecium. The entire 60 isolated LAB were tested for their acidity and bile tolerance, antimicrobial activity, and antibiotic sensitivity to test their potential probiotic activity. All 60 isolates were tolerant to different pH concentrations (2, 3, 4, 5, 6, 7, 8, 9, and 10) with different survival rates (%). The entire isolates were also tolerant to different bile salt concentrations (0.2, 0.4, 0.6, 0.8, 1, 2, and 3) with different bile resistance (%). All isolates have a different range of antimicrobial activity against Staphylococcus aureus, E. coli, and Salmonella typhimurium. The 60 isolates were almost sensitive to ampicillin, amoxicillin, and clarithromycin when different concentrations were used except some isolates of intermediate resistance. Only 6% of the isolates were resistant to clarithromycin at a concentration of 15 µg per disc.
format Online
Article
Text
id pubmed-10576644
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher Hindawi
record_format MEDLINE/PubMed
spelling pubmed-105766442023-10-15 Characterization of Potential Probiotic Activity of Lactic Acid Bacteria Isolated from Camel Colostrum by Biochemical and Molecular Methods Safi, Enas Haddad, Moawiya Hasan, Maen Al-Dalain, Sati Y. Proestos, Charalampos Siddiqui, Shahida A. Vet Med Int Research Article A total of 60 isolates of lactic acid bacteria (LAB) were isolated from Jordanian camel colostrum using biochemical and molecular methods. Two dominant species were identified, and they were Lactobacillus salivarius and Enterococcus faecium. The entire 60 isolated LAB were tested for their acidity and bile tolerance, antimicrobial activity, and antibiotic sensitivity to test their potential probiotic activity. All 60 isolates were tolerant to different pH concentrations (2, 3, 4, 5, 6, 7, 8, 9, and 10) with different survival rates (%). The entire isolates were also tolerant to different bile salt concentrations (0.2, 0.4, 0.6, 0.8, 1, 2, and 3) with different bile resistance (%). All isolates have a different range of antimicrobial activity against Staphylococcus aureus, E. coli, and Salmonella typhimurium. The 60 isolates were almost sensitive to ampicillin, amoxicillin, and clarithromycin when different concentrations were used except some isolates of intermediate resistance. Only 6% of the isolates were resistant to clarithromycin at a concentration of 15 µg per disc. Hindawi 2023-10-07 /pmc/articles/PMC10576644/ /pubmed/37841508 http://dx.doi.org/10.1155/2023/8334152 Text en Copyright © 2023 Enas Safi et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Safi, Enas
Haddad, Moawiya
Hasan, Maen
Al-Dalain, Sati Y.
Proestos, Charalampos
Siddiqui, Shahida A.
Characterization of Potential Probiotic Activity of Lactic Acid Bacteria Isolated from Camel Colostrum by Biochemical and Molecular Methods
title Characterization of Potential Probiotic Activity of Lactic Acid Bacteria Isolated from Camel Colostrum by Biochemical and Molecular Methods
title_full Characterization of Potential Probiotic Activity of Lactic Acid Bacteria Isolated from Camel Colostrum by Biochemical and Molecular Methods
title_fullStr Characterization of Potential Probiotic Activity of Lactic Acid Bacteria Isolated from Camel Colostrum by Biochemical and Molecular Methods
title_full_unstemmed Characterization of Potential Probiotic Activity of Lactic Acid Bacteria Isolated from Camel Colostrum by Biochemical and Molecular Methods
title_short Characterization of Potential Probiotic Activity of Lactic Acid Bacteria Isolated from Camel Colostrum by Biochemical and Molecular Methods
title_sort characterization of potential probiotic activity of lactic acid bacteria isolated from camel colostrum by biochemical and molecular methods
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10576644/
https://www.ncbi.nlm.nih.gov/pubmed/37841508
http://dx.doi.org/10.1155/2023/8334152
work_keys_str_mv AT safienas characterizationofpotentialprobioticactivityoflacticacidbacteriaisolatedfromcamelcolostrumbybiochemicalandmolecularmethods
AT haddadmoawiya characterizationofpotentialprobioticactivityoflacticacidbacteriaisolatedfromcamelcolostrumbybiochemicalandmolecularmethods
AT hasanmaen characterizationofpotentialprobioticactivityoflacticacidbacteriaisolatedfromcamelcolostrumbybiochemicalandmolecularmethods
AT aldalainsatiy characterizationofpotentialprobioticactivityoflacticacidbacteriaisolatedfromcamelcolostrumbybiochemicalandmolecularmethods
AT proestoscharalampos characterizationofpotentialprobioticactivityoflacticacidbacteriaisolatedfromcamelcolostrumbybiochemicalandmolecularmethods
AT siddiquishahidaa characterizationofpotentialprobioticactivityoflacticacidbacteriaisolatedfromcamelcolostrumbybiochemicalandmolecularmethods