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Quantitative analysis of gastrointestinal fluid absorption and secretion to estimate luminal fluid dynamics in rats

The drug absorption profile is dependent on the luminal drug concentration, which in turn is influenced by the gastrointestinal (GI) fluid dynamics. In the present study, therefore, we aimed to examine the luminal fluid dynamics by kinetically analyzing fluid absorption and secretion along the GI tr...

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Autores principales: Funai, Yuta, Ichijo, Kazuki, Suzuki, Satoru, Tateishi, Yuta, Inoue, Katsuhisa, Tamai, Ikumi, Shirasaka, Yoshiyuki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10576741/
https://www.ncbi.nlm.nih.gov/pubmed/37838772
http://dx.doi.org/10.1038/s41598-023-44742-y
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author Funai, Yuta
Ichijo, Kazuki
Suzuki, Satoru
Tateishi, Yuta
Inoue, Katsuhisa
Tamai, Ikumi
Shirasaka, Yoshiyuki
author_facet Funai, Yuta
Ichijo, Kazuki
Suzuki, Satoru
Tateishi, Yuta
Inoue, Katsuhisa
Tamai, Ikumi
Shirasaka, Yoshiyuki
author_sort Funai, Yuta
collection PubMed
description The drug absorption profile is dependent on the luminal drug concentration, which in turn is influenced by the gastrointestinal (GI) fluid dynamics. In the present study, therefore, we aimed to examine the luminal fluid dynamics by kinetically analyzing fluid absorption and secretion along the GI tract in rats using the in situ closed-loop technique with non-absorbable fluorescein isothiocyanate-dextran 4000 (FD-4) and tritium water labeling ([(3)H]water) under different osmotic conditions. We found that the luminal fluid volume in the jejunum and ileum, but not the colon, gradually decreased and reached a steady state. In contrast, [(3)H]water almost completely disappeared in all intestinal regions. Kinetic analysis revealed the following rank order for the rate constant of fluid secretion: jejunum > ileum > colon, whereas a negligible regional difference was observed in the rate constant of fluid absorption. Fluid secretion under an isosmotic condition (300 mOsm/kg) was higher than that at 0 mOsm/kg in all intestinal regions, though no significant changes in fluid absorption were observed. Thus, the fluid secretion process appears to be the major determinant of the regional differences in GI fluid dynamics. Our findings indicate that the luminal fluid volume is altered as a result of water ingestion, absorption, and secretion, and finally reaches an apparent steady state, which is regulated mainly by the process of fluid secretion.
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spelling pubmed-105767412023-10-16 Quantitative analysis of gastrointestinal fluid absorption and secretion to estimate luminal fluid dynamics in rats Funai, Yuta Ichijo, Kazuki Suzuki, Satoru Tateishi, Yuta Inoue, Katsuhisa Tamai, Ikumi Shirasaka, Yoshiyuki Sci Rep Article The drug absorption profile is dependent on the luminal drug concentration, which in turn is influenced by the gastrointestinal (GI) fluid dynamics. In the present study, therefore, we aimed to examine the luminal fluid dynamics by kinetically analyzing fluid absorption and secretion along the GI tract in rats using the in situ closed-loop technique with non-absorbable fluorescein isothiocyanate-dextran 4000 (FD-4) and tritium water labeling ([(3)H]water) under different osmotic conditions. We found that the luminal fluid volume in the jejunum and ileum, but not the colon, gradually decreased and reached a steady state. In contrast, [(3)H]water almost completely disappeared in all intestinal regions. Kinetic analysis revealed the following rank order for the rate constant of fluid secretion: jejunum > ileum > colon, whereas a negligible regional difference was observed in the rate constant of fluid absorption. Fluid secretion under an isosmotic condition (300 mOsm/kg) was higher than that at 0 mOsm/kg in all intestinal regions, though no significant changes in fluid absorption were observed. Thus, the fluid secretion process appears to be the major determinant of the regional differences in GI fluid dynamics. Our findings indicate that the luminal fluid volume is altered as a result of water ingestion, absorption, and secretion, and finally reaches an apparent steady state, which is regulated mainly by the process of fluid secretion. Nature Publishing Group UK 2023-10-14 /pmc/articles/PMC10576741/ /pubmed/37838772 http://dx.doi.org/10.1038/s41598-023-44742-y Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Funai, Yuta
Ichijo, Kazuki
Suzuki, Satoru
Tateishi, Yuta
Inoue, Katsuhisa
Tamai, Ikumi
Shirasaka, Yoshiyuki
Quantitative analysis of gastrointestinal fluid absorption and secretion to estimate luminal fluid dynamics in rats
title Quantitative analysis of gastrointestinal fluid absorption and secretion to estimate luminal fluid dynamics in rats
title_full Quantitative analysis of gastrointestinal fluid absorption and secretion to estimate luminal fluid dynamics in rats
title_fullStr Quantitative analysis of gastrointestinal fluid absorption and secretion to estimate luminal fluid dynamics in rats
title_full_unstemmed Quantitative analysis of gastrointestinal fluid absorption and secretion to estimate luminal fluid dynamics in rats
title_short Quantitative analysis of gastrointestinal fluid absorption and secretion to estimate luminal fluid dynamics in rats
title_sort quantitative analysis of gastrointestinal fluid absorption and secretion to estimate luminal fluid dynamics in rats
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10576741/
https://www.ncbi.nlm.nih.gov/pubmed/37838772
http://dx.doi.org/10.1038/s41598-023-44742-y
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