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Design and optimization of an improved qPCR assay for the detection of Histoplasma capsulatum

BACKGROUND: This study aimed at improving a real-time polymerase-chain-reaction (qPCR) assay for the detection of Histoplasma capsulatum, a fungal pathogen that can cause severe respiratory infections in humans, in clinical and soil samples. METHODS: Primer and probes were in-silico designed, in-sil...

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Detalles Bibliográficos
Autores principales: da Silva, Bianca Kelly Neves Izidro, Alves Cortez, Ana Cláudia, Oliveira, Luciana Aires, de Almeida, Juan Diego Ribeiro, de Souza, Érica Simplício, Cruz, Katia Santana, Jackisch Matsuura, Ani Beatriz, Ogusku, Mauricio Morishi, Solórzano Chavarría, Karolina Jeaneth, Baía-da-Silva, Djane, Bassat, Quique, Guimarães Lacerda, Marcus Vinícius, Frickmann, Hagen, de Souza, João Vicente Braga
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Akadémiai Kiadó 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10578137/
https://www.ncbi.nlm.nih.gov/pubmed/37738109
http://dx.doi.org/10.1556/1886.2023.00026