Expanding the genome editing toolbox of Saccharomyces cerevisiae with the endonuclease ErCas12a

ErCas12a is a class 2 type V CRISPR–Cas nuclease isolated from Eubacterium rectale with attractive fundamental characteristics, such as RNA self-processing capability, and lacks reach-through royalties typical for Cas nucleases. This study aims to develop a ErCas12a-mediated genome editing tool appl...

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Detalles Bibliográficos
Autores principales: Bennis, Nicole X, Anderson, Jonah P, Kok, Siebe M C, Daran, Jean-Marc G
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10583194/
https://www.ncbi.nlm.nih.gov/pubmed/37791490
http://dx.doi.org/10.1093/femsyr/foad043

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10583194/
https://www.ncbi.nlm.nih.gov/pubmed/37791490
http://dx.doi.org/10.1093/femsyr/foad043

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