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Expanding the genome editing toolbox of Saccharomyces cerevisiae with the endonuclease ErCas12a
ErCas12a is a class 2 type V CRISPR–Cas nuclease isolated from Eubacterium rectale with attractive fundamental characteristics, such as RNA self-processing capability, and lacks reach-through royalties typical for Cas nucleases. This study aims to develop a ErCas12a-mediated genome editing tool appl...
| Autores principales: | , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Oxford University Press
2023
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10583194/ https://www.ncbi.nlm.nih.gov/pubmed/37791490 http://dx.doi.org/10.1093/femsyr/foad043 |