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Self-assembly of promoter DNA and RNA Pol II machinery into transcriptionally active biomolecular condensates
Transcription in the nucleus occurs in a concentrated, dense environment, and no reasonable biochemical facsimile of this milieu exists. Such a biochemical environment would be important for further understanding transcriptional regulation. We describe here the formation of dense, transcriptionally...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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American Association for the Advancement of Science
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10584347/ https://www.ncbi.nlm.nih.gov/pubmed/37851801 http://dx.doi.org/10.1126/sciadv.adi4565 |
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author | Lewis, Brian A. Das, Subhendu Kumar Jha, Rajiv Kumar Levens, David |
author_facet | Lewis, Brian A. Das, Subhendu Kumar Jha, Rajiv Kumar Levens, David |
author_sort | Lewis, Brian A. |
collection | PubMed |
description | Transcription in the nucleus occurs in a concentrated, dense environment, and no reasonable biochemical facsimile of this milieu exists. Such a biochemical environment would be important for further understanding transcriptional regulation. We describe here the formation of dense, transcriptionally active bodies in vitro with only nuclear extracts and promoter DNA. These biomolecular condensates (BMCs) are 0.5 to 1 μm in diameter, have a macromolecular density of approximately 100 mg/ml, and are a consequence of a phase transition between promoter DNA and nuclear extract proteins. BMCs are physically associated with transcription as any disruption of one compromised the other. The BMCs contain RNA polymerase II and elongation factors, as well as factors necessary for BMC formation in vivo. We suggest that BMCs are representative of the in vivo nuclear environment and a more physiologically relevant manifestation of the preinitiation complex/elongation machinery. |
format | Online Article Text |
id | pubmed-10584347 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | American Association for the Advancement of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-105843472023-10-19 Self-assembly of promoter DNA and RNA Pol II machinery into transcriptionally active biomolecular condensates Lewis, Brian A. Das, Subhendu Kumar Jha, Rajiv Kumar Levens, David Sci Adv Biomedicine and Life Sciences Transcription in the nucleus occurs in a concentrated, dense environment, and no reasonable biochemical facsimile of this milieu exists. Such a biochemical environment would be important for further understanding transcriptional regulation. We describe here the formation of dense, transcriptionally active bodies in vitro with only nuclear extracts and promoter DNA. These biomolecular condensates (BMCs) are 0.5 to 1 μm in diameter, have a macromolecular density of approximately 100 mg/ml, and are a consequence of a phase transition between promoter DNA and nuclear extract proteins. BMCs are physically associated with transcription as any disruption of one compromised the other. The BMCs contain RNA polymerase II and elongation factors, as well as factors necessary for BMC formation in vivo. We suggest that BMCs are representative of the in vivo nuclear environment and a more physiologically relevant manifestation of the preinitiation complex/elongation machinery. American Association for the Advancement of Science 2023-10-18 /pmc/articles/PMC10584347/ /pubmed/37851801 http://dx.doi.org/10.1126/sciadv.adi4565 Text en Copyright © 2023 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution License 4.0 (CC BY). https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution license (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Biomedicine and Life Sciences Lewis, Brian A. Das, Subhendu Kumar Jha, Rajiv Kumar Levens, David Self-assembly of promoter DNA and RNA Pol II machinery into transcriptionally active biomolecular condensates |
title | Self-assembly of promoter DNA and RNA Pol II machinery into transcriptionally active biomolecular condensates |
title_full | Self-assembly of promoter DNA and RNA Pol II machinery into transcriptionally active biomolecular condensates |
title_fullStr | Self-assembly of promoter DNA and RNA Pol II machinery into transcriptionally active biomolecular condensates |
title_full_unstemmed | Self-assembly of promoter DNA and RNA Pol II machinery into transcriptionally active biomolecular condensates |
title_short | Self-assembly of promoter DNA and RNA Pol II machinery into transcriptionally active biomolecular condensates |
title_sort | self-assembly of promoter dna and rna pol ii machinery into transcriptionally active biomolecular condensates |
topic | Biomedicine and Life Sciences |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10584347/ https://www.ncbi.nlm.nih.gov/pubmed/37851801 http://dx.doi.org/10.1126/sciadv.adi4565 |
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