Knockout of CAFFEOYL-COA 3-O-METHYLTRANSFERASE 6/6L enhances the S/G ratio of lignin monomers and disease resistance in Nicotiana tabacum

BACKGROUND: Nicotiana tabacum is an important economic crop, which is widely planted in the world. Lignin is very important for maintaining the physiological and stress-resistant functions of tobacco. However, higher lignin content will produce lignin gas, which is not conducive to the formation of...

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Autores principales: Liu, Mingxin, Liu, Huayin, Zhang, Jianduo, Li, Cui, Li, Yinke, Yang, Guangyu, Xia, Tong, Huang, Haitao, Xu, Yong, Kong, Weisong, Hou, Bingzhu, Qi, Xiaoquan, Wang, Jin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10585270/
https://www.ncbi.nlm.nih.gov/pubmed/37868314
http://dx.doi.org/10.3389/fpls.2023.1216702
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author Liu, Mingxin
Liu, Huayin
Zhang, Jianduo
Li, Cui
Li, Yinke
Yang, Guangyu
Xia, Tong
Huang, Haitao
Xu, Yong
Kong, Weisong
Hou, Bingzhu
Qi, Xiaoquan
Wang, Jin
author_facet Liu, Mingxin
Liu, Huayin
Zhang, Jianduo
Li, Cui
Li, Yinke
Yang, Guangyu
Xia, Tong
Huang, Haitao
Xu, Yong
Kong, Weisong
Hou, Bingzhu
Qi, Xiaoquan
Wang, Jin
author_sort Liu, Mingxin
collection PubMed
description BACKGROUND: Nicotiana tabacum is an important economic crop, which is widely planted in the world. Lignin is very important for maintaining the physiological and stress-resistant functions of tobacco. However, higher lignin content will produce lignin gas, which is not conducive to the formation of tobacco quality. To date, how to precisely fine-tune lignin content or composition remains unclear. RESULTS: Here, we annotated and screened 14 CCoAOMTs in Nicotiana tabacum and obtained homozygous double mutants of CCoAOMT6 and CCoAOMT6L through CRSIPR/Cas9 technology. The phenotype showed that the double mutants have better growth than the wild type whereas the S/G ratio increased and the total sugar decreased. Resistance against the pathogen test and the extract inhibition test showed that the transgenic tobacco has stronger resistance to tobacco bacterial wilt and brown spot disease, which are infected by Ralstonia solanacearum and Alternaria alternata, respectively. The combined analysis of metabolome and transcriptome in the leaves and roots suggested that the changes of phenylpropane and terpene metabolism are mainly responsible for these phenotypes. Furthermore, the molecular docking indicated that the upregulated metabolites, such as soyasaponin Bb, improve the disease resistance due to highly stable binding with tyrosyl-tRNA synthetase targets in Ralstonia solanacearum and Alternaria alternata. CONCLUSIONS: CAFFEOYL-COA 3-O-METHYLTRANSFERASE 6/6L can regulate the S/G ratio of lignin monomers and may affect tobacco bacterial wilt and brown spot disease resistance by disturbing phenylpropane and terpene metabolism in leaves and roots of Nicotiana tabacum, such as soyasaponin Bb.
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spelling pubmed-105852702023-10-20 Knockout of CAFFEOYL-COA 3-O-METHYLTRANSFERASE 6/6L enhances the S/G ratio of lignin monomers and disease resistance in Nicotiana tabacum Liu, Mingxin Liu, Huayin Zhang, Jianduo Li, Cui Li, Yinke Yang, Guangyu Xia, Tong Huang, Haitao Xu, Yong Kong, Weisong Hou, Bingzhu Qi, Xiaoquan Wang, Jin Front Plant Sci Plant Science BACKGROUND: Nicotiana tabacum is an important economic crop, which is widely planted in the world. Lignin is very important for maintaining the physiological and stress-resistant functions of tobacco. However, higher lignin content will produce lignin gas, which is not conducive to the formation of tobacco quality. To date, how to precisely fine-tune lignin content or composition remains unclear. RESULTS: Here, we annotated and screened 14 CCoAOMTs in Nicotiana tabacum and obtained homozygous double mutants of CCoAOMT6 and CCoAOMT6L through CRSIPR/Cas9 technology. The phenotype showed that the double mutants have better growth than the wild type whereas the S/G ratio increased and the total sugar decreased. Resistance against the pathogen test and the extract inhibition test showed that the transgenic tobacco has stronger resistance to tobacco bacterial wilt and brown spot disease, which are infected by Ralstonia solanacearum and Alternaria alternata, respectively. The combined analysis of metabolome and transcriptome in the leaves and roots suggested that the changes of phenylpropane and terpene metabolism are mainly responsible for these phenotypes. Furthermore, the molecular docking indicated that the upregulated metabolites, such as soyasaponin Bb, improve the disease resistance due to highly stable binding with tyrosyl-tRNA synthetase targets in Ralstonia solanacearum and Alternaria alternata. CONCLUSIONS: CAFFEOYL-COA 3-O-METHYLTRANSFERASE 6/6L can regulate the S/G ratio of lignin monomers and may affect tobacco bacterial wilt and brown spot disease resistance by disturbing phenylpropane and terpene metabolism in leaves and roots of Nicotiana tabacum, such as soyasaponin Bb. Frontiers Media S.A. 2023-10-05 /pmc/articles/PMC10585270/ /pubmed/37868314 http://dx.doi.org/10.3389/fpls.2023.1216702 Text en Copyright © 2023 Liu, Liu, Zhang, Li, Li, Yang, Xia, Huang, Xu, Kong, Hou, Qi and Wang https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Liu, Mingxin
Liu, Huayin
Zhang, Jianduo
Li, Cui
Li, Yinke
Yang, Guangyu
Xia, Tong
Huang, Haitao
Xu, Yong
Kong, Weisong
Hou, Bingzhu
Qi, Xiaoquan
Wang, Jin
Knockout of CAFFEOYL-COA 3-O-METHYLTRANSFERASE 6/6L enhances the S/G ratio of lignin monomers and disease resistance in Nicotiana tabacum
title Knockout of CAFFEOYL-COA 3-O-METHYLTRANSFERASE 6/6L enhances the S/G ratio of lignin monomers and disease resistance in Nicotiana tabacum
title_full Knockout of CAFFEOYL-COA 3-O-METHYLTRANSFERASE 6/6L enhances the S/G ratio of lignin monomers and disease resistance in Nicotiana tabacum
title_fullStr Knockout of CAFFEOYL-COA 3-O-METHYLTRANSFERASE 6/6L enhances the S/G ratio of lignin monomers and disease resistance in Nicotiana tabacum
title_full_unstemmed Knockout of CAFFEOYL-COA 3-O-METHYLTRANSFERASE 6/6L enhances the S/G ratio of lignin monomers and disease resistance in Nicotiana tabacum
title_short Knockout of CAFFEOYL-COA 3-O-METHYLTRANSFERASE 6/6L enhances the S/G ratio of lignin monomers and disease resistance in Nicotiana tabacum
title_sort knockout of caffeoyl-coa 3-o-methyltransferase 6/6l enhances the s/g ratio of lignin monomers and disease resistance in nicotiana tabacum
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10585270/
https://www.ncbi.nlm.nih.gov/pubmed/37868314
http://dx.doi.org/10.3389/fpls.2023.1216702
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