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Amplification-Based CRISPR/Cas12a Biosensor Targeting the COX1 Gene for Specific Detection of Porcine DNA

[Image: see text] We propose a CRISPR/Cas12a-mediated recombinase polymerase amplification (RPA) detection method that combines RPA with Cas12a cleavage for the detection of halal food adulteration, which is of global concern, particularly for Muslim consumers. We optimized the reagent concentration...

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Detalles Bibliográficos
Autores principales: Janudin, Arifah A. S., Kurup, Chitra P., Chee, Lim Ya, Mohd-Naim, Noor F., Ahmed, Minhaz U.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2023
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10586177/
https://www.ncbi.nlm.nih.gov/pubmed/37867655
http://dx.doi.org/10.1021/acsomega.3c04473