CRISPR/Cas9-based depletion of 16S ribosomal RNA improves library complexity of single-cell RNA-sequencing in planarians

BACKGROUND: Single-cell RNA-sequencing (scRNA-seq) relies on PCR amplification to retrieve information from vanishingly small amounts of starting material. To selectively enrich mRNA from abundant non-polyadenylated transcripts, poly(A) selection is a key step during library preparation. However, so...

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Detalles Bibliográficos
Autores principales: Wang, Kuang-Tse, Adler, Carolyn E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2023
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10588366/
https://www.ncbi.nlm.nih.gov/pubmed/37864134
http://dx.doi.org/10.1186/s12864-023-09724-4

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10588366/
https://www.ncbi.nlm.nih.gov/pubmed/37864134
http://dx.doi.org/10.1186/s12864-023-09724-4

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