Identification of a novel family B DNA polymerase from Enterococcus phage IME199 and its overproduction in Escherichia coli BL21(DE3)

BACKGROUND: Identification and characterization of novel, faithful and processive DNA polymerases is a driving force in the development of DNA amplification methods. Purification of proteins from natural phages is often time-consuming, cumbersome and low yielding. Escherichia coli is a host bacteriu...

Descripción completa

Detalles Bibliográficos
Autores principales: Han, Pengjun, Fan, Huahao, Tong, Yigang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2023
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10590003/
https://www.ncbi.nlm.nih.gov/pubmed/37865739
http://dx.doi.org/10.1186/s12934-023-02228-6

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10590003/
https://www.ncbi.nlm.nih.gov/pubmed/37865739
http://dx.doi.org/10.1186/s12934-023-02228-6

Ejemplares similares