Improved dsRNA isolation and purification method validated by viral dsRNA detection using novel primers in Saccharomyces cerevisiae

Accurate genomic sequencing demands high-quality double-stranded RNA (dsRNA). Existing methods for dsRNA extraction from yeast, fungi, and plants primarily rely on cellulose, suitable only for small volume extractions, or the time-consuming lithium chloride precipitation. To streamline the tradition...

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Detalles Bibliográficos
Autores principales: Cardoso, Fernando M.H., Elias, Alexandre, Pereira, Inês, Maurício, Isabel, Matos, Olga
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Biochemistry, Genetics and Molecular Biology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10591000/
https://www.ncbi.nlm.nih.gov/pubmed/37876828
http://dx.doi.org/10.1016/j.mex.2023.102435

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10591000/
https://www.ncbi.nlm.nih.gov/pubmed/37876828
http://dx.doi.org/10.1016/j.mex.2023.102435

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