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Development of nanoRibo-seq enables study of regulated translation by cortical neuron subtypes, showing uORF translation in synaptic-axonal genes

Investigation of translation in rare cell types or subcellular contexts is challenging due to large input requirements for standard approaches. Here, we present “nanoRibo-seq” an optimized approach using 10(2)- to 10(3)-fold less input material than bulk approaches. nanoRibo-seq exhibits rigorous qu...

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Detalles Bibliográficos
Autores principales: Froberg, John E., Durak, Omer, Macklis, Jeffrey D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10591829/
https://www.ncbi.nlm.nih.gov/pubmed/37624698
http://dx.doi.org/10.1016/j.celrep.2023.112995