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Development of nanoRibo-seq enables study of regulated translation by cortical neuron subtypes, showing uORF translation in synaptic-axonal genes
Investigation of translation in rare cell types or subcellular contexts is challenging due to large input requirements for standard approaches. Here, we present “nanoRibo-seq” an optimized approach using 10(2)- to 10(3)-fold less input material than bulk approaches. nanoRibo-seq exhibits rigorous qu...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10591829/ https://www.ncbi.nlm.nih.gov/pubmed/37624698 http://dx.doi.org/10.1016/j.celrep.2023.112995 |