Oleic acid differentially affects de novo lipogenesis in adipocytes and hepatocytes

Lipogenesis is a vital but often dysregulated metabolic pathway. We report super-resolution multiplexed vibrational imaging of lipogenesis rates and pathways using isotopically labelled oleic acid and glucose as probes in live adipocytes and hepatocytes. These findings suggest oleic acid inhibits de...

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Autores principales: Castillo, Hannah B., Shuster, Sydney O., Tarekegn, Lydia H., Davis, Caitlin M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory 2023
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10592964/
https://www.ncbi.nlm.nih.gov/pubmed/37873279
http://dx.doi.org/10.1101/2023.10.04.560581
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author Castillo, Hannah B.
Shuster, Sydney O.
Tarekegn, Lydia H.
Davis, Caitlin M.
author_facet Castillo, Hannah B.
Shuster, Sydney O.
Tarekegn, Lydia H.
Davis, Caitlin M.
author_sort Castillo, Hannah B.
collection PubMed
description Lipogenesis is a vital but often dysregulated metabolic pathway. We report super-resolution multiplexed vibrational imaging of lipogenesis rates and pathways using isotopically labelled oleic acid and glucose as probes in live adipocytes and hepatocytes. These findings suggest oleic acid inhibits de novo lipogenesis (DNL), but not total lipogenesis, in hepatocytes. No significant effect is seen in adipocytes. These differential effects may be due to alternate regulation of DNL between cell types and could help explain the complicated role oleic acid plays in metabolism.
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spelling pubmed-105929642023-10-24 Oleic acid differentially affects de novo lipogenesis in adipocytes and hepatocytes Castillo, Hannah B. Shuster, Sydney O. Tarekegn, Lydia H. Davis, Caitlin M. bioRxiv Article Lipogenesis is a vital but often dysregulated metabolic pathway. We report super-resolution multiplexed vibrational imaging of lipogenesis rates and pathways using isotopically labelled oleic acid and glucose as probes in live adipocytes and hepatocytes. These findings suggest oleic acid inhibits de novo lipogenesis (DNL), but not total lipogenesis, in hepatocytes. No significant effect is seen in adipocytes. These differential effects may be due to alternate regulation of DNL between cell types and could help explain the complicated role oleic acid plays in metabolism. Cold Spring Harbor Laboratory 2023-10-06 /pmc/articles/PMC10592964/ /pubmed/37873279 http://dx.doi.org/10.1101/2023.10.04.560581 Text en https://creativecommons.org/licenses/by-nc-nd/4.0/This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which allows reusers to copy and distribute the material in any medium or format in unadapted form only, for noncommercial purposes only, and only so long as attribution is given to the creator.
spellingShingle Article
Castillo, Hannah B.
Shuster, Sydney O.
Tarekegn, Lydia H.
Davis, Caitlin M.
Oleic acid differentially affects de novo lipogenesis in adipocytes and hepatocytes
title Oleic acid differentially affects de novo lipogenesis in adipocytes and hepatocytes
title_full Oleic acid differentially affects de novo lipogenesis in adipocytes and hepatocytes
title_fullStr Oleic acid differentially affects de novo lipogenesis in adipocytes and hepatocytes
title_full_unstemmed Oleic acid differentially affects de novo lipogenesis in adipocytes and hepatocytes
title_short Oleic acid differentially affects de novo lipogenesis in adipocytes and hepatocytes
title_sort oleic acid differentially affects de novo lipogenesis in adipocytes and hepatocytes
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10592964/
https://www.ncbi.nlm.nih.gov/pubmed/37873279
http://dx.doi.org/10.1101/2023.10.04.560581
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