Cleavage of delta and epsilon tubulin PCR products is observed upon PCR purification

In this study, we report an unusual phenomenon of the self-cleavage of purified PCR products of codon-optimized Chlamydomonas reinhardtii delta tubulin ( uni3 ) and epsilon tubulin ( bld2 ) genes through an unknown mechanism. Our studies revealed that intact PCR products for both these genes could b...

Descripción completa

Detalles Bibliográficos
Autores principales: Parab, Prasad, Iyer, Jyoti, Johannes, Tyler W
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Caltech Library 2023
Materias:
New Finding
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10594130/
https://www.ncbi.nlm.nih.gov/pubmed/37881246
http://dx.doi.org/10.17912/micropub.biology.000980

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10594130/
https://www.ncbi.nlm.nih.gov/pubmed/37881246
http://dx.doi.org/10.17912/micropub.biology.000980

Ejemplares similares