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Cleavage of delta and epsilon tubulin PCR products is observed upon PCR purification

In this study, we report an unusual phenomenon of the self-cleavage of purified PCR products of codon-optimized Chlamydomonas reinhardtii delta tubulin ( uni3 ) and epsilon tubulin ( bld2 ) genes through an unknown mechanism. Our studies revealed that intact PCR products for both these genes could b...

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Detalles Bibliográficos
Autores principales:	Parab, Prasad, Iyer, Jyoti, Johannes, Tyler W
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Caltech Library 2023
Materias:	New Finding
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10594130/ https://www.ncbi.nlm.nih.gov/pubmed/37881246 http://dx.doi.org/10.17912/micropub.biology.000980

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