Protocol for auxin-inducible depletion of the RNA-binding protein PTBP1 in mouse embryonic stem cells

Inducible degradation of proteins of interest provides a powerful approach for functional studies. Here, we present a protocol for tightly controlled depletion of the RNA-binding protein PTBP1 in mouse embryonic stem cells (ESCs). We describe steps for establishing an ESC line expressing doxycycline...

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Detalles Bibliográficos
Autores principales: Kainov, Yaroslav, Zhuravskaya, Anna, Makeyev, Eugene V.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10594634/
https://www.ncbi.nlm.nih.gov/pubmed/37862173
http://dx.doi.org/10.1016/j.xpro.2023.102644

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10594634/
https://www.ncbi.nlm.nih.gov/pubmed/37862173
http://dx.doi.org/10.1016/j.xpro.2023.102644

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