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Complex Dependence of Escherichia coli-based Cell-Free Expression on Sonication Energy During Lysis
[Image: see text] Cell lysis—by sonication or bead beating, for example—is a key step in preparing extracts for cell-free expression systems. To create high protein-production capacity extracts, standard practice is to lyse cells sufficiently to thoroughly disrupt the membrane and thus extract expre...
| Autores principales: | , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
American Chemical Society
2023
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10594866/ https://www.ncbi.nlm.nih.gov/pubmed/37725792 http://dx.doi.org/10.1021/acssynbio.3c00312 |