Nalaganje...
Synthetic Gene Circuits Combining CRISPR Interference and CRISPR Activation in E. coli: Importance of Equal Guide RNA Binding Affinities to Avoid Context-Dependent Effects
[Image: see text] Gene expression control based on clustered regularly interspaced short palindromic repeats (CRISPR) has emerged as a powerful approach for constructing synthetic gene circuits. While the use of CRISPR interference (CRISPRi) is already well-established in prokaryotic circuits, CRISP...
| Autores principales: | , , , , , |
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| Format: | Online Artículo Texto |
| Jezik: | English |
| Izdano: |
American Chemical Society
2023
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| Online dostop: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10594877/ https://www.ncbi.nlm.nih.gov/pubmed/37813387 http://dx.doi.org/10.1021/acssynbio.3c00375 |