Synthetic Gene Circuits Combining CRISPR Interference and CRISPR Activation in E. coli: Importance of Equal Guide RNA Binding Affinities to Avoid Context-Dependent Effects

[Image: see text] Gene expression control based on clustered regularly interspaced short palindromic repeats (CRISPR) has emerged as a powerful approach for constructing synthetic gene circuits. While the use of CRISPR interference (CRISPRi) is already well-established in prokaryotic circuits, CRISP...

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Autores principales: Barbier, Içvara, Kusumawardhani, Hadiastri, Chauhan, Lakshya, Harlapur, Pradyumna Vinod, Jolly, Mohit Kumar, Schaerli, Yolanda
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2023
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10594877/
https://www.ncbi.nlm.nih.gov/pubmed/37813387
http://dx.doi.org/10.1021/acssynbio.3c00375

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