Elimination of bla(KPC−2)-mediated carbapenem resistance in Escherichia coli by CRISPR-Cas9 system

OBJECTIVE: The purpose of this study is to re-sensitive bacteria to carbapenemases and reduce the transmission of the bla(KPC−2) gene by curing the bla(KPC−2)-harboring plasmid of carbapenem-resistant using the CRISPR-Cas9 system. METHODS: The single guide RNA (sgRNA) specifically targeted to the bl...

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Detalles Bibliográficos
Autores principales: Tao, Shuan, Chen, Huimin, Li, Na, Fang, Yewei, Zhang, He, Xu, Yao, Chen, Luyan, Liang, Wei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2023
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10601263/
https://www.ncbi.nlm.nih.gov/pubmed/37884864
http://dx.doi.org/10.1186/s12866-023-03058-7

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10601263/
https://www.ncbi.nlm.nih.gov/pubmed/37884864
http://dx.doi.org/10.1186/s12866-023-03058-7

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