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Elimination of bla(KPC−2)-mediated carbapenem resistance in Escherichia coli by CRISPR-Cas9 system

OBJECTIVE: The purpose of this study is to re-sensitive bacteria to carbapenemases and reduce the transmission of the bla(KPC−2) gene by curing the bla(KPC−2)-harboring plasmid of carbapenem-resistant using the CRISPR-Cas9 system. METHODS: The single guide RNA (sgRNA) specifically targeted to the bl...

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Detalles Bibliográficos
Autores principales:	Tao, Shuan, Chen, Huimin, Li, Na, Fang, Yewei, Zhang, He, Xu, Yao, Chen, Luyan, Liang, Wei
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BioMed Central 2023
Materias:	Research
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10601263/ https://www.ncbi.nlm.nih.gov/pubmed/37884864 http://dx.doi.org/10.1186/s12866-023-03058-7

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