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Efficient Large DNA Fragment Knock-in by Long dsDNA with 3′-Overhangs Mediated CRISPR Knock-in (LOCK) in Mammalian Cells

An efficient and precise genome-editing approach is in high demand in any molecular biology or cell biology laboratory worldwide. However, despite a recent rapid progress in the toolbox tailored for precise genome-editing, including the base editors and prime editors, there is still a need for a cos...

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Detalles Bibliográficos
Autores principales: Han, Wenjie, Liang, Haojun, Bao, Jianqiang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Bio-Protocol 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10603260/
https://www.ncbi.nlm.nih.gov/pubmed/37900108
http://dx.doi.org/10.21769/BioProtoc.4853