Serial-section Electron Tomography and Quantitative Analysis of Microtubule Organization in 3D-reconstructed Mitotic Spindles

For the analysis of cellular architecture during mitosis, nanometer resolution is needed to visualize the organization of microtubules in spindles. Here, we present a detailed protocol that can be used to produce 3D reconstructions of whole mitotic spindles in cells grown in culture. For this, we at...

Descripción completa

Detalles Bibliográficos
Autores principales: Kiewisz, Robert, Baum, Daniel, Müller-Reichert, Thomas, Fabig, Gunar
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Bio-Protocol 2023
Materias:
Methods Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10603263/
https://www.ncbi.nlm.nih.gov/pubmed/37900106
http://dx.doi.org/10.21769/BioProtoc.4849

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10603263/
https://www.ncbi.nlm.nih.gov/pubmed/37900106
http://dx.doi.org/10.21769/BioProtoc.4849

Ejemplares similares