Cargando…

Preventive Action of Beta-Carotene against the Indoxyl Sulfate-Induced Renal Dysfunction in Male Adult Zebrafish via Regulations of Mitochondrial Inflammatory and β-Carotene Oxygenase-2 Actions

Indoxyl sulfate (IS) is a metabolic byproduct of indole metabolism. IS readily interacts with the mitochondrial redox metabolism, leading to altered renal function. The β-carotene oxygenase-2 (BCO2) enzyme converts carotenoids to intermediate products. However, the role of β-carotene (BC) in IS-indu...

Descripción completa

Detalles Bibliográficos
Autores principales: Muthuraman, Arunachalam, Sayem, Abu Sadat Md., Meenakshisundaram, Sakthiganapathi, Ali, Nemat, Ahmad, Sheikh F., AlAsmari, Abdullah F., Nishat, Shamama, Lim, Khian Giap, Paramaswaran, Yamunna
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10603961/
https://www.ncbi.nlm.nih.gov/pubmed/37893028
http://dx.doi.org/10.3390/biomedicines11102654
_version_ 1785126721421312000
author Muthuraman, Arunachalam
Sayem, Abu Sadat Md.
Meenakshisundaram, Sakthiganapathi
Ali, Nemat
Ahmad, Sheikh F.
AlAsmari, Abdullah F.
Nishat, Shamama
Lim, Khian Giap
Paramaswaran, Yamunna
author_facet Muthuraman, Arunachalam
Sayem, Abu Sadat Md.
Meenakshisundaram, Sakthiganapathi
Ali, Nemat
Ahmad, Sheikh F.
AlAsmari, Abdullah F.
Nishat, Shamama
Lim, Khian Giap
Paramaswaran, Yamunna
author_sort Muthuraman, Arunachalam
collection PubMed
description Indoxyl sulfate (IS) is a metabolic byproduct of indole metabolism. IS readily interacts with the mitochondrial redox metabolism, leading to altered renal function. The β-carotene oxygenase-2 (BCO2) enzyme converts carotenoids to intermediate products. However, the role of β-carotene (BC) in IS-induced renal dysfunction in zebrafish and their modulatory action on BCO2 and mitochondrial inflammations have not been explored yet. Hence, the present study is designed to investigate the role of BC in the attenuation of IS-induced renal dysfunction via regulations of mitochondrial redox balance by BCO2 actions. Renal dysfunction was induced by exposure to IS (10 mg/L/hour/day) for 4 weeks. BC (50 and 100 mg/L/hour/day) and coenzyme Q10 (CoQ10; 20 mg/L/hour/day) were added before IS exposure. BC attenuated the IS-induced increase in blood urea nitrogen (BUN) and creatinine concentrations, adenosine triphosphate (ATP), and complex I activity levels, and the reduction of renal mitochondrial biomarkers, i.e., BCO2, superoxide dismutase-2 (SOD2), glutathione peroxidase-1 (GPX1), reduced and oxidized glutathione (GSH/GSSG) ratio, and carbonylated proteins. Moreover, renal histopathological changes were analyzed by the eosin and hematoxylin staining method. As a result, the administration of BC attenuated the IS-induced renal damage via the regulation of mitochondrial function.
format Online
Article
Text
id pubmed-10603961
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-106039612023-10-28 Preventive Action of Beta-Carotene against the Indoxyl Sulfate-Induced Renal Dysfunction in Male Adult Zebrafish via Regulations of Mitochondrial Inflammatory and β-Carotene Oxygenase-2 Actions Muthuraman, Arunachalam Sayem, Abu Sadat Md. Meenakshisundaram, Sakthiganapathi Ali, Nemat Ahmad, Sheikh F. AlAsmari, Abdullah F. Nishat, Shamama Lim, Khian Giap Paramaswaran, Yamunna Biomedicines Article Indoxyl sulfate (IS) is a metabolic byproduct of indole metabolism. IS readily interacts with the mitochondrial redox metabolism, leading to altered renal function. The β-carotene oxygenase-2 (BCO2) enzyme converts carotenoids to intermediate products. However, the role of β-carotene (BC) in IS-induced renal dysfunction in zebrafish and their modulatory action on BCO2 and mitochondrial inflammations have not been explored yet. Hence, the present study is designed to investigate the role of BC in the attenuation of IS-induced renal dysfunction via regulations of mitochondrial redox balance by BCO2 actions. Renal dysfunction was induced by exposure to IS (10 mg/L/hour/day) for 4 weeks. BC (50 and 100 mg/L/hour/day) and coenzyme Q10 (CoQ10; 20 mg/L/hour/day) were added before IS exposure. BC attenuated the IS-induced increase in blood urea nitrogen (BUN) and creatinine concentrations, adenosine triphosphate (ATP), and complex I activity levels, and the reduction of renal mitochondrial biomarkers, i.e., BCO2, superoxide dismutase-2 (SOD2), glutathione peroxidase-1 (GPX1), reduced and oxidized glutathione (GSH/GSSG) ratio, and carbonylated proteins. Moreover, renal histopathological changes were analyzed by the eosin and hematoxylin staining method. As a result, the administration of BC attenuated the IS-induced renal damage via the regulation of mitochondrial function. MDPI 2023-09-27 /pmc/articles/PMC10603961/ /pubmed/37893028 http://dx.doi.org/10.3390/biomedicines11102654 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Muthuraman, Arunachalam
Sayem, Abu Sadat Md.
Meenakshisundaram, Sakthiganapathi
Ali, Nemat
Ahmad, Sheikh F.
AlAsmari, Abdullah F.
Nishat, Shamama
Lim, Khian Giap
Paramaswaran, Yamunna
Preventive Action of Beta-Carotene against the Indoxyl Sulfate-Induced Renal Dysfunction in Male Adult Zebrafish via Regulations of Mitochondrial Inflammatory and β-Carotene Oxygenase-2 Actions
title Preventive Action of Beta-Carotene against the Indoxyl Sulfate-Induced Renal Dysfunction in Male Adult Zebrafish via Regulations of Mitochondrial Inflammatory and β-Carotene Oxygenase-2 Actions
title_full Preventive Action of Beta-Carotene against the Indoxyl Sulfate-Induced Renal Dysfunction in Male Adult Zebrafish via Regulations of Mitochondrial Inflammatory and β-Carotene Oxygenase-2 Actions
title_fullStr Preventive Action of Beta-Carotene against the Indoxyl Sulfate-Induced Renal Dysfunction in Male Adult Zebrafish via Regulations of Mitochondrial Inflammatory and β-Carotene Oxygenase-2 Actions
title_full_unstemmed Preventive Action of Beta-Carotene against the Indoxyl Sulfate-Induced Renal Dysfunction in Male Adult Zebrafish via Regulations of Mitochondrial Inflammatory and β-Carotene Oxygenase-2 Actions
title_short Preventive Action of Beta-Carotene against the Indoxyl Sulfate-Induced Renal Dysfunction in Male Adult Zebrafish via Regulations of Mitochondrial Inflammatory and β-Carotene Oxygenase-2 Actions
title_sort preventive action of beta-carotene against the indoxyl sulfate-induced renal dysfunction in male adult zebrafish via regulations of mitochondrial inflammatory and β-carotene oxygenase-2 actions
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10603961/
https://www.ncbi.nlm.nih.gov/pubmed/37893028
http://dx.doi.org/10.3390/biomedicines11102654
work_keys_str_mv AT muthuramanarunachalam preventiveactionofbetacaroteneagainsttheindoxylsulfateinducedrenaldysfunctioninmaleadultzebrafishviaregulationsofmitochondrialinflammatoryandbcaroteneoxygenase2actions
AT sayemabusadatmd preventiveactionofbetacaroteneagainsttheindoxylsulfateinducedrenaldysfunctioninmaleadultzebrafishviaregulationsofmitochondrialinflammatoryandbcaroteneoxygenase2actions
AT meenakshisundaramsakthiganapathi preventiveactionofbetacaroteneagainsttheindoxylsulfateinducedrenaldysfunctioninmaleadultzebrafishviaregulationsofmitochondrialinflammatoryandbcaroteneoxygenase2actions
AT alinemat preventiveactionofbetacaroteneagainsttheindoxylsulfateinducedrenaldysfunctioninmaleadultzebrafishviaregulationsofmitochondrialinflammatoryandbcaroteneoxygenase2actions
AT ahmadsheikhf preventiveactionofbetacaroteneagainsttheindoxylsulfateinducedrenaldysfunctioninmaleadultzebrafishviaregulationsofmitochondrialinflammatoryandbcaroteneoxygenase2actions
AT alasmariabdullahf preventiveactionofbetacaroteneagainsttheindoxylsulfateinducedrenaldysfunctioninmaleadultzebrafishviaregulationsofmitochondrialinflammatoryandbcaroteneoxygenase2actions
AT nishatshamama preventiveactionofbetacaroteneagainsttheindoxylsulfateinducedrenaldysfunctioninmaleadultzebrafishviaregulationsofmitochondrialinflammatoryandbcaroteneoxygenase2actions
AT limkhiangiap preventiveactionofbetacaroteneagainsttheindoxylsulfateinducedrenaldysfunctioninmaleadultzebrafishviaregulationsofmitochondrialinflammatoryandbcaroteneoxygenase2actions
AT paramaswaranyamunna preventiveactionofbetacaroteneagainsttheindoxylsulfateinducedrenaldysfunctioninmaleadultzebrafishviaregulationsofmitochondrialinflammatoryandbcaroteneoxygenase2actions