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Direct Enzyme Engineering of B Family DNA Polymerases for Biotechnological Approaches
DNA-dependent DNA polymerases have been intensively studied for more than 60 years and underlie numerous biotechnological and diagnostic applications. In vitro, DNA polymerases are used for DNA manipulations, including cloning, PCR, site-directed mutagenesis, sequencing, and others. Understanding th...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10604792/ https://www.ncbi.nlm.nih.gov/pubmed/37892880 http://dx.doi.org/10.3390/bioengineering10101150 |