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Direct Enzyme Engineering of B Family DNA Polymerases for Biotechnological Approaches

DNA-dependent DNA polymerases have been intensively studied for more than 60 years and underlie numerous biotechnological and diagnostic applications. In vitro, DNA polymerases are used for DNA manipulations, including cloning, PCR, site-directed mutagenesis, sequencing, and others. Understanding th...

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Detalles Bibliográficos
Autores principales: Kuznetsova, Aleksandra A., Kuznetsov, Nikita A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10604792/
https://www.ncbi.nlm.nih.gov/pubmed/37892880
http://dx.doi.org/10.3390/bioengineering10101150