A Simple Validated Method for the Estimation of Pepsin Activity in Microtiter Array for the INFOGEST Protocol

The INFOGEST protocol has been widely used as a static in-vitro simulation of gastrointestinal food digestion for bioaccessibility assessments on bioactive compounds. The standardization of the activity of several enzymes, such as pepsin, via UV-spectrophotometry of digested hemoglobin at 280 nm is...

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Detalles Bibliográficos
Autores principales: Ramm, Maximiliano, Alarcón-Zapata, Bárbara, Monsalves, Juan, Bustamante, Luis
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10606326/
https://www.ncbi.nlm.nih.gov/pubmed/37893746
http://dx.doi.org/10.3390/foods12203851

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10606326/
https://www.ncbi.nlm.nih.gov/pubmed/37893746
http://dx.doi.org/10.3390/foods12203851

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