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A Simple Validated Method for the Estimation of Pepsin Activity in Microtiter Array for the INFOGEST Protocol
The INFOGEST protocol has been widely used as a static in-vitro simulation of gastrointestinal food digestion for bioaccessibility assessments on bioactive compounds. The standardization of the activity of several enzymes, such as pepsin, via UV-spectrophotometry of digested hemoglobin at 280 nm is...
| Autores principales: | , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
MDPI
2023
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10606326/ https://www.ncbi.nlm.nih.gov/pubmed/37893746 http://dx.doi.org/10.3390/foods12203851 |