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Histone Abundance Quantification via Flow Cytometry of Htb2-GFP Allows Easy Monitoring of Cell Cycle Perturbations in Living Yeast Cells, Comparable to Standard DNA Staining

Assaying changes in the amount of DNA in single cells is a well-established method for studying the effects of various perturbations on the cell cycle. A drawback of this method is the need for a fixation procedure that does not allow for in vivo study nor simultaneous monitoring of additional param...

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Detalles Bibliográficos
Autores principales: Kulakova, Maria V., Ghazy, Eslam S. M. O., Ryabov, Fedor, Stanishevskiy, Yaroslav M., Agaphonov, Michael O., Alexandrov, Alexander I.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10608138/
https://www.ncbi.nlm.nih.gov/pubmed/37888289
http://dx.doi.org/10.3390/jof9101033