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Histone Abundance Quantification via Flow Cytometry of Htb2-GFP Allows Easy Monitoring of Cell Cycle Perturbations in Living Yeast Cells, Comparable to Standard DNA Staining
Assaying changes in the amount of DNA in single cells is a well-established method for studying the effects of various perturbations on the cell cycle. A drawback of this method is the need for a fixation procedure that does not allow for in vivo study nor simultaneous monitoring of additional param...
Autores principales: | Kulakova, Maria V., Ghazy, Eslam S. M. O., Ryabov, Fedor, Stanishevskiy, Yaroslav M., Agaphonov, Michael O., Alexandrov, Alexander I. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10608138/ https://www.ncbi.nlm.nih.gov/pubmed/37888289 http://dx.doi.org/10.3390/jof9101033 |
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