Generation of a Recombinant scFv against Deoxycholic Acid and Its Conversion to a Quenchbody for One-Step Immunoassay

Development of a rapid detection method for deoxycholic acid (DCA) is crucial for its diagnosis in the early stages of inflammation and cancer. In this study, we expressed a soluble recombinant anti-DCA single-chain variable fragment (scFv) in Escherichia coli. To convert scFv into a Quenchbody (Q-b...

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Detalles Bibliográficos
Autores principales: Ueda, Hiroshi, Jeong, Hee-Jin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Technical Note
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10608803/
https://www.ncbi.nlm.nih.gov/pubmed/37888022
http://dx.doi.org/10.3390/mps6050090

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10608803/
https://www.ncbi.nlm.nih.gov/pubmed/37888022
http://dx.doi.org/10.3390/mps6050090

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