A NitroPure Nitrocellulose Membrane-Based Grapevine Virus Sampling Kit: Development and Deployment to Survey Japanese Vineyards and Nurseries

We developed a NitroPure Nitrocellulose (NPN) membrane-based method for sampling and storing grapevine sap for grapevine virus detection. We devised an efficient nucleic acid extraction method for the NPN membrane, resulting in 100% amplification success for grapevine leafroll-associated virus 2 (GL...

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Detalles Bibliográficos
Autores principales: Nita, Mizuho, Jones, Taylor, McHenry, Diana, Bush, Elizabeth, Oliver, Charlotte, Kawaguchi, Akira, Nita, Akiko, Katori, Miyuki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10612103/
https://www.ncbi.nlm.nih.gov/pubmed/37896878
http://dx.doi.org/10.3390/v15102102

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10612103/
https://www.ncbi.nlm.nih.gov/pubmed/37896878
http://dx.doi.org/10.3390/v15102102

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