Analysis of the gut microbiota in children with gastroesophageal reflux disease using metagenomics and metabolomics

BACKGROUND: There is no direct evidence of gut microbiota disturbance in children with gastroesophageal reflux disease (GERD). This study aimed to provide direct evidence and a comprehensive understanding of gut microbiota disturbance in children with GERD through combined metagenomic and metabolomi...

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Autores principales: Ye, Xiaolin, Yu, Feihong, Zhou, Jin, Zhao, Chunna, Wu, Jie, Ni, Xin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10613033/
https://www.ncbi.nlm.nih.gov/pubmed/37900308
http://dx.doi.org/10.3389/fcimb.2023.1267192
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author Ye, Xiaolin
Yu, Feihong
Zhou, Jin
Zhao, Chunna
Wu, Jie
Ni, Xin
author_facet Ye, Xiaolin
Yu, Feihong
Zhou, Jin
Zhao, Chunna
Wu, Jie
Ni, Xin
author_sort Ye, Xiaolin
collection PubMed
description BACKGROUND: There is no direct evidence of gut microbiota disturbance in children with gastroesophageal reflux disease (GERD). This study aimed to provide direct evidence and a comprehensive understanding of gut microbiota disturbance in children with GERD through combined metagenomic and metabolomic analysis. METHODS: 30 children with GERD and 30 healthy controls (HCs) were continuously enrolled, and the demographic and clinical characteristics of the subjects were collected. First, 16S rRNA sequencing was used to evaluate differences in the gut microbiota between children with GERD and HC group, and 10 children with GERD and 10 children in the HC group were selected for metagenomic analysis. Nontargeted metabolomic analysis was performed using liquid chromatography/mass spectrometry (LC/MS), and metagenomic and metabolomic data were analyzed together. RESULTS: There were significant differences in the gut microbiota diversity and composition between children with GERD and HCs. The dominant bacteria in children with GERD were Proteobacteria and Bacteroidota. At the species level, the top three core bacterial groups were Bacteroides stercoris, Bacteroides vulgatus and Alistipes putredinis. The main differential pathways were identified to be related to energy, amino acid, vitamin, carbohydrate and lipid metabolism. LC/MS detected 288 different metabolites in the positive and negative ion modes between children with GERD and HCs, which were mainly involved in arachidonic acid (AA), tyrosine, glutathione and caffeine metabolism. CONCLUSION: This study provides new evidence of the pathogenesis of GERD. There are significant differences in the gut microbiota, metabolites and metabolic pathways between HCs and children with GERD, and the differences in metabolites are related to specific changes in bacterial abundance. In the future, GERD may be treated by targeting specific bacteria related to AA metabolism.
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spelling pubmed-106130332023-10-29 Analysis of the gut microbiota in children with gastroesophageal reflux disease using metagenomics and metabolomics Ye, Xiaolin Yu, Feihong Zhou, Jin Zhao, Chunna Wu, Jie Ni, Xin Front Cell Infect Microbiol Cellular and Infection Microbiology BACKGROUND: There is no direct evidence of gut microbiota disturbance in children with gastroesophageal reflux disease (GERD). This study aimed to provide direct evidence and a comprehensive understanding of gut microbiota disturbance in children with GERD through combined metagenomic and metabolomic analysis. METHODS: 30 children with GERD and 30 healthy controls (HCs) were continuously enrolled, and the demographic and clinical characteristics of the subjects were collected. First, 16S rRNA sequencing was used to evaluate differences in the gut microbiota between children with GERD and HC group, and 10 children with GERD and 10 children in the HC group were selected for metagenomic analysis. Nontargeted metabolomic analysis was performed using liquid chromatography/mass spectrometry (LC/MS), and metagenomic and metabolomic data were analyzed together. RESULTS: There were significant differences in the gut microbiota diversity and composition between children with GERD and HCs. The dominant bacteria in children with GERD were Proteobacteria and Bacteroidota. At the species level, the top three core bacterial groups were Bacteroides stercoris, Bacteroides vulgatus and Alistipes putredinis. The main differential pathways were identified to be related to energy, amino acid, vitamin, carbohydrate and lipid metabolism. LC/MS detected 288 different metabolites in the positive and negative ion modes between children with GERD and HCs, which were mainly involved in arachidonic acid (AA), tyrosine, glutathione and caffeine metabolism. CONCLUSION: This study provides new evidence of the pathogenesis of GERD. There are significant differences in the gut microbiota, metabolites and metabolic pathways between HCs and children with GERD, and the differences in metabolites are related to specific changes in bacterial abundance. In the future, GERD may be treated by targeting specific bacteria related to AA metabolism. Frontiers Media S.A. 2023-10-13 /pmc/articles/PMC10613033/ /pubmed/37900308 http://dx.doi.org/10.3389/fcimb.2023.1267192 Text en Copyright © 2023 Ye, Yu, Zhou, Zhao, Wu and Ni https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cellular and Infection Microbiology
Ye, Xiaolin
Yu, Feihong
Zhou, Jin
Zhao, Chunna
Wu, Jie
Ni, Xin
Analysis of the gut microbiota in children with gastroesophageal reflux disease using metagenomics and metabolomics
title Analysis of the gut microbiota in children with gastroesophageal reflux disease using metagenomics and metabolomics
title_full Analysis of the gut microbiota in children with gastroesophageal reflux disease using metagenomics and metabolomics
title_fullStr Analysis of the gut microbiota in children with gastroesophageal reflux disease using metagenomics and metabolomics
title_full_unstemmed Analysis of the gut microbiota in children with gastroesophageal reflux disease using metagenomics and metabolomics
title_short Analysis of the gut microbiota in children with gastroesophageal reflux disease using metagenomics and metabolomics
title_sort analysis of the gut microbiota in children with gastroesophageal reflux disease using metagenomics and metabolomics
topic Cellular and Infection Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10613033/
https://www.ncbi.nlm.nih.gov/pubmed/37900308
http://dx.doi.org/10.3389/fcimb.2023.1267192
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