Molecular Time Capsules Enable Transcriptomic Recording in Living Cells

Live-cell transcriptomic recording can help reveal hidden cellular states that precede phenotypic transformation. Here we demonstrate the use of protein-based encapsulation for preserving samples of cytoplasmic RNAs inside living cells. These molecular time capsules (MTCs) can be induced to create t...

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Detalles Bibliográficos
Autores principales: Parker, Mirae, Rubien, Jack, McCormick, Dylan, Li, Gene-Wei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory 2023
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10614764/
https://www.ncbi.nlm.nih.gov/pubmed/37905077
http://dx.doi.org/10.1101/2023.10.12.562053
Descripción
Sumario:Live-cell transcriptomic recording can help reveal hidden cellular states that precede phenotypic transformation. Here we demonstrate the use of protein-based encapsulation for preserving samples of cytoplasmic RNAs inside living cells. These molecular time capsules (MTCs) can be induced to create time-stamped transcriptome snapshots, preserve RNAs after cellular transitions, and enable retrospective investigations of gene expression programs that drive distinct developmental trajectories. MTCs also open the possibility to uncover transcriptomes in difficult-to-reach conditions.

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