Molecular Time Capsules Enable Transcriptomic Recording in Living Cells

Live-cell transcriptomic recording can help reveal hidden cellular states that precede phenotypic transformation. Here we demonstrate the use of protein-based encapsulation for preserving samples of cytoplasmic RNAs inside living cells. These molecular time capsules (MTCs) can be induced to create t...

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Autores principales: Parker, Mirae, Rubien, Jack, McCormick, Dylan, Li, Gene-Wei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory 2023
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10614764/
https://www.ncbi.nlm.nih.gov/pubmed/37905077
http://dx.doi.org/10.1101/2023.10.12.562053
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author Parker, Mirae
Rubien, Jack
McCormick, Dylan
Li, Gene-Wei
author_facet Parker, Mirae
Rubien, Jack
McCormick, Dylan
Li, Gene-Wei
author_sort Parker, Mirae
collection PubMed
description Live-cell transcriptomic recording can help reveal hidden cellular states that precede phenotypic transformation. Here we demonstrate the use of protein-based encapsulation for preserving samples of cytoplasmic RNAs inside living cells. These molecular time capsules (MTCs) can be induced to create time-stamped transcriptome snapshots, preserve RNAs after cellular transitions, and enable retrospective investigations of gene expression programs that drive distinct developmental trajectories. MTCs also open the possibility to uncover transcriptomes in difficult-to-reach conditions.
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spelling pubmed-106147642023-10-31 Molecular Time Capsules Enable Transcriptomic Recording in Living Cells Parker, Mirae Rubien, Jack McCormick, Dylan Li, Gene-Wei bioRxiv Article Live-cell transcriptomic recording can help reveal hidden cellular states that precede phenotypic transformation. Here we demonstrate the use of protein-based encapsulation for preserving samples of cytoplasmic RNAs inside living cells. These molecular time capsules (MTCs) can be induced to create time-stamped transcriptome snapshots, preserve RNAs after cellular transitions, and enable retrospective investigations of gene expression programs that drive distinct developmental trajectories. MTCs also open the possibility to uncover transcriptomes in difficult-to-reach conditions. Cold Spring Harbor Laboratory 2023-10-16 /pmc/articles/PMC10614764/ /pubmed/37905077 http://dx.doi.org/10.1101/2023.10.12.562053 Text en https://creativecommons.org/licenses/by-nc-nd/4.0/This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which allows reusers to copy and distribute the material in any medium or format in unadapted form only, for noncommercial purposes only, and only so long as attribution is given to the creator.
spellingShingle Article
Parker, Mirae
Rubien, Jack
McCormick, Dylan
Li, Gene-Wei
Molecular Time Capsules Enable Transcriptomic Recording in Living Cells
title Molecular Time Capsules Enable Transcriptomic Recording in Living Cells
title_full Molecular Time Capsules Enable Transcriptomic Recording in Living Cells
title_fullStr Molecular Time Capsules Enable Transcriptomic Recording in Living Cells
title_full_unstemmed Molecular Time Capsules Enable Transcriptomic Recording in Living Cells
title_short Molecular Time Capsules Enable Transcriptomic Recording in Living Cells
title_sort molecular time capsules enable transcriptomic recording in living cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10614764/
https://www.ncbi.nlm.nih.gov/pubmed/37905077
http://dx.doi.org/10.1101/2023.10.12.562053
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AT ligenewei moleculartimecapsulesenabletranscriptomicrecordinginlivingcells

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