Diagnostic utility of N-terminal TMPP labels for unambiguous identification of clipped sites in therapeutic proteins

Protein therapeutics are susceptible to clipping via enzymatic and nonenzymatic mechanisms that create neo-N-termini. Typically, neo-N-termini are identified by chemical derivatization of the N-terminal amine with (N-Succinimidyloxycarbonylmethyl)tris(2,4,6-trimethoxyphenyl)phosphonium bromide (TMPP...

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Detalles Bibliográficos
Autores principales: Gunawardena, Harsha P., Jayatilake, Meth M., Brelsford, Jeffery D., Nanda, Hirsh
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2023
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10616084/
https://www.ncbi.nlm.nih.gov/pubmed/37903854
http://dx.doi.org/10.1038/s41598-023-45446-z

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10616084/
https://www.ncbi.nlm.nih.gov/pubmed/37903854
http://dx.doi.org/10.1038/s41598-023-45446-z

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