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IDO1 facilitates esophageal carcinoma progression by driving the direct binding of NF-κB and CXCL10
Esophageal carcinoma (EC), one of the most lethal human malignancies, lacks effective targeted therapies. Indoleamine 2,3-dioxygenase 1 (IDO1) plays a key role in a variety of cancers, but its role and mechanism in EC are still unclear. Immunohistochemistry and qRT-PCR were used to analyze the expre...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10616276/ https://www.ncbi.nlm.nih.gov/pubmed/37903782 http://dx.doi.org/10.1038/s41420-023-01689-3 |
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author | Yao, Wenjian Cui, Xiaohai Peng, Haodong Zhang, Yongkang Jia, Xiangbo Wu, Sen Zhao, Jian |
author_facet | Yao, Wenjian Cui, Xiaohai Peng, Haodong Zhang, Yongkang Jia, Xiangbo Wu, Sen Zhao, Jian |
author_sort | Yao, Wenjian |
collection | PubMed |
description | Esophageal carcinoma (EC), one of the most lethal human malignancies, lacks effective targeted therapies. Indoleamine 2,3-dioxygenase 1 (IDO1) plays a key role in a variety of cancers, but its role and mechanism in EC are still unclear. Immunohistochemistry and qRT-PCR were used to analyze the expression of IDO1 in EC, and the prognostic value of IDO1 in EC was evaluated by Kaplan-Meier test. The in vitro and in vivo function loss/acquisition tests were performed to evaluate the biological effects of IDO1 in EC. The mechanism of action of IDO1-regulation EC was explored through Firefly luciferase & Renilla luciferase activity reporter, chromatin immunoprecipitation (ChIP) and immunofluorescence (IF) assays. Clinically, IDO1 expression was abnormally elevated in EC and positively correlated with overall survival. Functionally, IDO1 was contributed to the proliferation and migration of EC cells. Mechanically, IDO1 regulated the expression of chemokine C-X-C ligand 10 (CXCL10) by promoting the entry of NF-κB into the nucleus to combine with the promoter of CXCL10. Consistently, IDO1 facilitated EC progression may dependent on the presence of CXCL10. Moreover, NF-κB alleviated the inhibitory effect of IDO1 knockdown on EC. IDO1 drove the progression of EC by directly binding NF-κB and CXCL10, the finding that may provide an effective theoretical basis for precise therapies for EC. |
format | Online Article Text |
id | pubmed-10616276 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-106162762023-11-01 IDO1 facilitates esophageal carcinoma progression by driving the direct binding of NF-κB and CXCL10 Yao, Wenjian Cui, Xiaohai Peng, Haodong Zhang, Yongkang Jia, Xiangbo Wu, Sen Zhao, Jian Cell Death Discov Article Esophageal carcinoma (EC), one of the most lethal human malignancies, lacks effective targeted therapies. Indoleamine 2,3-dioxygenase 1 (IDO1) plays a key role in a variety of cancers, but its role and mechanism in EC are still unclear. Immunohistochemistry and qRT-PCR were used to analyze the expression of IDO1 in EC, and the prognostic value of IDO1 in EC was evaluated by Kaplan-Meier test. The in vitro and in vivo function loss/acquisition tests were performed to evaluate the biological effects of IDO1 in EC. The mechanism of action of IDO1-regulation EC was explored through Firefly luciferase & Renilla luciferase activity reporter, chromatin immunoprecipitation (ChIP) and immunofluorescence (IF) assays. Clinically, IDO1 expression was abnormally elevated in EC and positively correlated with overall survival. Functionally, IDO1 was contributed to the proliferation and migration of EC cells. Mechanically, IDO1 regulated the expression of chemokine C-X-C ligand 10 (CXCL10) by promoting the entry of NF-κB into the nucleus to combine with the promoter of CXCL10. Consistently, IDO1 facilitated EC progression may dependent on the presence of CXCL10. Moreover, NF-κB alleviated the inhibitory effect of IDO1 knockdown on EC. IDO1 drove the progression of EC by directly binding NF-κB and CXCL10, the finding that may provide an effective theoretical basis for precise therapies for EC. Nature Publishing Group UK 2023-10-31 /pmc/articles/PMC10616276/ /pubmed/37903782 http://dx.doi.org/10.1038/s41420-023-01689-3 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Yao, Wenjian Cui, Xiaohai Peng, Haodong Zhang, Yongkang Jia, Xiangbo Wu, Sen Zhao, Jian IDO1 facilitates esophageal carcinoma progression by driving the direct binding of NF-κB and CXCL10 |
title | IDO1 facilitates esophageal carcinoma progression by driving the direct binding of NF-κB and CXCL10 |
title_full | IDO1 facilitates esophageal carcinoma progression by driving the direct binding of NF-κB and CXCL10 |
title_fullStr | IDO1 facilitates esophageal carcinoma progression by driving the direct binding of NF-κB and CXCL10 |
title_full_unstemmed | IDO1 facilitates esophageal carcinoma progression by driving the direct binding of NF-κB and CXCL10 |
title_short | IDO1 facilitates esophageal carcinoma progression by driving the direct binding of NF-κB and CXCL10 |
title_sort | ido1 facilitates esophageal carcinoma progression by driving the direct binding of nf-κb and cxcl10 |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10616276/ https://www.ncbi.nlm.nih.gov/pubmed/37903782 http://dx.doi.org/10.1038/s41420-023-01689-3 |
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