Protocol for assessing translation in living Drosophila imaginal discs by O-propargyl-puromycin incorporation

Translation is a fundamental process of cellular behavior. Here, we present a protocol for measuring translation in Drosophila epithelial tissues using O-propargyl-puromycin (OPP), a puromycin derivative. We detail steps for larval dissection, OPP incorporation, fixation, OPP labeling, immunostainin...

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Detalles Bibliográficos
Autores principales: Kiparaki, Marianthi, Baker, Nicholas E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10616417/
https://www.ncbi.nlm.nih.gov/pubmed/37862174
http://dx.doi.org/10.1016/j.xpro.2023.102653

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10616417/
https://www.ncbi.nlm.nih.gov/pubmed/37862174
http://dx.doi.org/10.1016/j.xpro.2023.102653

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