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Bidirectional Interactions between Green Tea (GT) Polyphenols and Human Gut Bacteria
Green tea (GT) polyphenols undergo extensive metabolism within gastrointestinal tract (GIT), where their derivatives compounds potentially modulate the gut microbiome. This biotransformation process involves a cascade of exclusive gut microbial enzymes which chemically modify the GT polyphenols infl...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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The Korean Society for Microbiology and Biotechnology
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10619559/ https://www.ncbi.nlm.nih.gov/pubmed/37435870 http://dx.doi.org/10.4014/jmb.2306.06014 |
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author | Choi, Se Rin Lee, Hyunji Singh, Digar Cho, Donghyun Chung, Jin-Oh Roh, Jong-Hwa Kim, Wan-Gi Lee, Choong Hwan |
author_facet | Choi, Se Rin Lee, Hyunji Singh, Digar Cho, Donghyun Chung, Jin-Oh Roh, Jong-Hwa Kim, Wan-Gi Lee, Choong Hwan |
author_sort | Choi, Se Rin |
collection | PubMed |
description | Green tea (GT) polyphenols undergo extensive metabolism within gastrointestinal tract (GIT), where their derivatives compounds potentially modulate the gut microbiome. This biotransformation process involves a cascade of exclusive gut microbial enzymes which chemically modify the GT polyphenols influencing both their bioactivity and bioavailability in host. Herein, we examined the in vitro interactions between 37 different human gut microbiota and the GT polyphenols. UHPLC-LTQ-Orbitrap-MS/MS analysis of the culture broth extracts unravel that genera Adlercreutzia, Eggerthella and Lactiplantibacillus plantarum KACC11451 promoted C-ring opening reaction in GT catechins. In addition, L. plantarum also hydrolyzed catechin galloyl esters to produce gallic acid and pyrogallol, and also converted flavonoid glycosides to their aglycone derivatives. Biotransformation of GT polyphenols into derivative compounds enhanced their antioxidant bioactivities in culture broth extracts. Considering the effects of GT polyphenols on specific growth rates of gut bacteria, we noted that GT polyphenols and their derivate compounds inhibited most species in phylum Actinobacteria, Bacteroides, and Firmicutes except genus Lactobacillus. The present study delineates the likely mechanisms involved in the metabolism and bioavailability of GT polyphenols upon exposure to gut microbiota. Further, widening this workflow to understand the metabolism of various other dietary polyphenols can unravel their biotransformation mechanisms and associated functions in human GIT. |
format | Online Article Text |
id | pubmed-10619559 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | The Korean Society for Microbiology and Biotechnology |
record_format | MEDLINE/PubMed |
spelling | pubmed-106195592023-11-02 Bidirectional Interactions between Green Tea (GT) Polyphenols and Human Gut Bacteria Choi, Se Rin Lee, Hyunji Singh, Digar Cho, Donghyun Chung, Jin-Oh Roh, Jong-Hwa Kim, Wan-Gi Lee, Choong Hwan J Microbiol Biotechnol Research article Green tea (GT) polyphenols undergo extensive metabolism within gastrointestinal tract (GIT), where their derivatives compounds potentially modulate the gut microbiome. This biotransformation process involves a cascade of exclusive gut microbial enzymes which chemically modify the GT polyphenols influencing both their bioactivity and bioavailability in host. Herein, we examined the in vitro interactions between 37 different human gut microbiota and the GT polyphenols. UHPLC-LTQ-Orbitrap-MS/MS analysis of the culture broth extracts unravel that genera Adlercreutzia, Eggerthella and Lactiplantibacillus plantarum KACC11451 promoted C-ring opening reaction in GT catechins. In addition, L. plantarum also hydrolyzed catechin galloyl esters to produce gallic acid and pyrogallol, and also converted flavonoid glycosides to their aglycone derivatives. Biotransformation of GT polyphenols into derivative compounds enhanced their antioxidant bioactivities in culture broth extracts. Considering the effects of GT polyphenols on specific growth rates of gut bacteria, we noted that GT polyphenols and their derivate compounds inhibited most species in phylum Actinobacteria, Bacteroides, and Firmicutes except genus Lactobacillus. The present study delineates the likely mechanisms involved in the metabolism and bioavailability of GT polyphenols upon exposure to gut microbiota. Further, widening this workflow to understand the metabolism of various other dietary polyphenols can unravel their biotransformation mechanisms and associated functions in human GIT. The Korean Society for Microbiology and Biotechnology 2023-10-28 2023-07-12 /pmc/articles/PMC10619559/ /pubmed/37435870 http://dx.doi.org/10.4014/jmb.2306.06014 Text en Copyright © 2023 by the authors. Licensee KMB https://creativecommons.org/licenses/by/4.0/This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/) |
spellingShingle | Research article Choi, Se Rin Lee, Hyunji Singh, Digar Cho, Donghyun Chung, Jin-Oh Roh, Jong-Hwa Kim, Wan-Gi Lee, Choong Hwan Bidirectional Interactions between Green Tea (GT) Polyphenols and Human Gut Bacteria |
title | Bidirectional Interactions between Green Tea (GT) Polyphenols and Human Gut Bacteria |
title_full | Bidirectional Interactions between Green Tea (GT) Polyphenols and Human Gut Bacteria |
title_fullStr | Bidirectional Interactions between Green Tea (GT) Polyphenols and Human Gut Bacteria |
title_full_unstemmed | Bidirectional Interactions between Green Tea (GT) Polyphenols and Human Gut Bacteria |
title_short | Bidirectional Interactions between Green Tea (GT) Polyphenols and Human Gut Bacteria |
title_sort | bidirectional interactions between green tea (gt) polyphenols and human gut bacteria |
topic | Research article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10619559/ https://www.ncbi.nlm.nih.gov/pubmed/37435870 http://dx.doi.org/10.4014/jmb.2306.06014 |
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