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Liver sinusoidal endothelial cells show reduced scavenger function and downregulation of Fc gamma receptor IIb, yet maintain a preserved fenestration in the Glmp(gt/gt) mouse model of slowly progressing liver fibrosis

Liver sinusoidal endothelial cells (LSECs) are fenestrated endothelial cells with a unique, high endocytic clearance capacity for blood-borne waste macromolecules and colloids. This LSEC scavenger function has been insufficiently characterized in liver disease. The Glmp(gt/gt) mouse lacks expression...

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Autores principales: Antwi, Milton Boaheng, Dumitriu, Gianina, Simón-Santamaria, Jaione, Romano, Javier Sánchez, Li, Ruomei, Smedsrød, Bård, Vik, Anders, Eskild, Winnie, Sørensen, Karen Kristine
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10619817/
https://www.ncbi.nlm.nih.gov/pubmed/37910485
http://dx.doi.org/10.1371/journal.pone.0293526
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author Antwi, Milton Boaheng
Dumitriu, Gianina
Simón-Santamaria, Jaione
Romano, Javier Sánchez
Li, Ruomei
Smedsrød, Bård
Vik, Anders
Eskild, Winnie
Sørensen, Karen Kristine
author_facet Antwi, Milton Boaheng
Dumitriu, Gianina
Simón-Santamaria, Jaione
Romano, Javier Sánchez
Li, Ruomei
Smedsrød, Bård
Vik, Anders
Eskild, Winnie
Sørensen, Karen Kristine
author_sort Antwi, Milton Boaheng
collection PubMed
description Liver sinusoidal endothelial cells (LSECs) are fenestrated endothelial cells with a unique, high endocytic clearance capacity for blood-borne waste macromolecules and colloids. This LSEC scavenger function has been insufficiently characterized in liver disease. The Glmp(gt/gt) mouse lacks expression of a subunit of the MFSD1/GLMP lysosomal membrane protein transporter complex, is born normal, but soon develops chronic, mild hepatocyte injury, leading to slowly progressing periportal liver fibrosis, and splenomegaly. This study examined how LSEC scavenger function and morphology are affected in the Glmp(gt/gt) model. FITC-labelled formaldehyde-treated serum albumin (FITC-FSA), a model ligand for LSEC scavenger receptors was administered intravenously into Glmp(gt/gt) mice, aged 4 months (peak of liver inflammation), 9–10 month, and age-matched Glmp(wt/wt) mice. Organs were harvested for light and electron microscopy, quantitative image analysis of ligand uptake, collagen accumulation, LSEC ultrastructure, and endocytosis receptor expression (also examined by qPCR and western blot). In both age groups, the Glmp(gt/gt) mice showed multifocal liver injury and fibrosis. The uptake of FITC-FSA in LSECs was significantly reduced in Glmp(gt/gt) compared to wild-type mice. Expression of LSEC receptors stabilin-1 (Stab1), and mannose receptor (Mcr1) was almost similar in liver of Glmp(gt/gt) mice and age-matched controls. At the same time, immunostaining revealed differences in the stabilin-1 expression pattern in sinusoids and accumulation of stabilin-1-positive macrophages in Glmp(gt/gt) liver. FcγRIIb (Fcgr2b), which mediates LSEC endocytosis of soluble immune complexes was widely and significantly downregulated in Glmp(gt/gt) liver. Despite increased collagen in space of Disse, LSECs of Glmp(gt/gt) mice showed well-preserved fenestrae organized in sieve plates but the frequency of holes >400 nm in diameter was increased, especially in areas with hepatocyte damage. In both genotypes, FITC-FSA also distributed to endothelial cells of spleen and bone marrow sinusoids, suggesting that these locations may function as possible compensatory sites of clearance of blood-borne scavenger receptor ligands in liver fibrosis.
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spelling pubmed-106198172023-11-02 Liver sinusoidal endothelial cells show reduced scavenger function and downregulation of Fc gamma receptor IIb, yet maintain a preserved fenestration in the Glmp(gt/gt) mouse model of slowly progressing liver fibrosis Antwi, Milton Boaheng Dumitriu, Gianina Simón-Santamaria, Jaione Romano, Javier Sánchez Li, Ruomei Smedsrød, Bård Vik, Anders Eskild, Winnie Sørensen, Karen Kristine PLoS One Research Article Liver sinusoidal endothelial cells (LSECs) are fenestrated endothelial cells with a unique, high endocytic clearance capacity for blood-borne waste macromolecules and colloids. This LSEC scavenger function has been insufficiently characterized in liver disease. The Glmp(gt/gt) mouse lacks expression of a subunit of the MFSD1/GLMP lysosomal membrane protein transporter complex, is born normal, but soon develops chronic, mild hepatocyte injury, leading to slowly progressing periportal liver fibrosis, and splenomegaly. This study examined how LSEC scavenger function and morphology are affected in the Glmp(gt/gt) model. FITC-labelled formaldehyde-treated serum albumin (FITC-FSA), a model ligand for LSEC scavenger receptors was administered intravenously into Glmp(gt/gt) mice, aged 4 months (peak of liver inflammation), 9–10 month, and age-matched Glmp(wt/wt) mice. Organs were harvested for light and electron microscopy, quantitative image analysis of ligand uptake, collagen accumulation, LSEC ultrastructure, and endocytosis receptor expression (also examined by qPCR and western blot). In both age groups, the Glmp(gt/gt) mice showed multifocal liver injury and fibrosis. The uptake of FITC-FSA in LSECs was significantly reduced in Glmp(gt/gt) compared to wild-type mice. Expression of LSEC receptors stabilin-1 (Stab1), and mannose receptor (Mcr1) was almost similar in liver of Glmp(gt/gt) mice and age-matched controls. At the same time, immunostaining revealed differences in the stabilin-1 expression pattern in sinusoids and accumulation of stabilin-1-positive macrophages in Glmp(gt/gt) liver. FcγRIIb (Fcgr2b), which mediates LSEC endocytosis of soluble immune complexes was widely and significantly downregulated in Glmp(gt/gt) liver. Despite increased collagen in space of Disse, LSECs of Glmp(gt/gt) mice showed well-preserved fenestrae organized in sieve plates but the frequency of holes >400 nm in diameter was increased, especially in areas with hepatocyte damage. In both genotypes, FITC-FSA also distributed to endothelial cells of spleen and bone marrow sinusoids, suggesting that these locations may function as possible compensatory sites of clearance of blood-borne scavenger receptor ligands in liver fibrosis. Public Library of Science 2023-11-01 /pmc/articles/PMC10619817/ /pubmed/37910485 http://dx.doi.org/10.1371/journal.pone.0293526 Text en © 2023 Antwi et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Antwi, Milton Boaheng
Dumitriu, Gianina
Simón-Santamaria, Jaione
Romano, Javier Sánchez
Li, Ruomei
Smedsrød, Bård
Vik, Anders
Eskild, Winnie
Sørensen, Karen Kristine
Liver sinusoidal endothelial cells show reduced scavenger function and downregulation of Fc gamma receptor IIb, yet maintain a preserved fenestration in the Glmp(gt/gt) mouse model of slowly progressing liver fibrosis
title Liver sinusoidal endothelial cells show reduced scavenger function and downregulation of Fc gamma receptor IIb, yet maintain a preserved fenestration in the Glmp(gt/gt) mouse model of slowly progressing liver fibrosis
title_full Liver sinusoidal endothelial cells show reduced scavenger function and downregulation of Fc gamma receptor IIb, yet maintain a preserved fenestration in the Glmp(gt/gt) mouse model of slowly progressing liver fibrosis
title_fullStr Liver sinusoidal endothelial cells show reduced scavenger function and downregulation of Fc gamma receptor IIb, yet maintain a preserved fenestration in the Glmp(gt/gt) mouse model of slowly progressing liver fibrosis
title_full_unstemmed Liver sinusoidal endothelial cells show reduced scavenger function and downregulation of Fc gamma receptor IIb, yet maintain a preserved fenestration in the Glmp(gt/gt) mouse model of slowly progressing liver fibrosis
title_short Liver sinusoidal endothelial cells show reduced scavenger function and downregulation of Fc gamma receptor IIb, yet maintain a preserved fenestration in the Glmp(gt/gt) mouse model of slowly progressing liver fibrosis
title_sort liver sinusoidal endothelial cells show reduced scavenger function and downregulation of fc gamma receptor iib, yet maintain a preserved fenestration in the glmp(gt/gt) mouse model of slowly progressing liver fibrosis
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10619817/
https://www.ncbi.nlm.nih.gov/pubmed/37910485
http://dx.doi.org/10.1371/journal.pone.0293526
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