CRISPR/Cas9 bioluminescence-based assay for monitoring CFTR trafficking to the plasma membrane

CFTR is a membrane protein that functions as an ion channel. Mutations that disrupt its biosynthesis, trafficking or function cause cystic fibrosis (CF). Here, we present a novel in vitro model system prepared using CRISPR/Cas9 genome editing with endogenously expressed WT-CFTR tagged with a HiBiT p...

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Detalles Bibliográficos
Autores principales: Ondra, Martin, Lenart, Lukas, Centorame, Amanda, Dumut, Daciana C, He, Alexander, Zaidi, Syeda Sadaf Zehra, Hanrahan, John W, De Sanctis, Juan Bautista, Radzioch, Danuta, Hajduch, Marian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Life Science Alliance LLC 2023
Materias:
Methods
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10622324/
https://www.ncbi.nlm.nih.gov/pubmed/37918963
http://dx.doi.org/10.26508/lsa.202302045

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10622324/
https://www.ncbi.nlm.nih.gov/pubmed/37918963
http://dx.doi.org/10.26508/lsa.202302045

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