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Fine-tuning CRISPR/Cas9 gene editing in common bean (Phaseolus vulgaris L.) using a hairy root transformation system and in silico prediction models

The stable transformation of common bean is a challenging and time-consuming process. Although CRISPR/Cas9 has revolutionized gene editing with its high efficiency and specificity, the performance of the system can be affected by multiple factors, such as sgRNA specificity and effectiveness, and the...

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Detalles Bibliográficos
Autores principales: de Koning, Ramon, Daryanavard, Hana, Garmyn, Joyce, Kiekens, Raphaël, Toili, Mary Esther Muyoka, Angenon, Geert
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10623320/
https://www.ncbi.nlm.nih.gov/pubmed/37929181
http://dx.doi.org/10.3389/fpls.2023.1233418