A duplex droplet digital PCR assay for absolute quantification and characterization of long self-amplifying RNA

Self-amplifying messenger ribonucleic acid (saRNA) provides extended expression of genes of interest by encoding an alphavirus-derived RNA replicase and thus is 2–3 times larger than conventional messenger RNA. However, quality assessment of long RNA transcripts is challenging using standard techniq...

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Detalles Bibliográficos
Autores principales: Casmil, Irafasha C., Huang, Cynthia, Blakney, Anna K.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2023
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10624827/
https://www.ncbi.nlm.nih.gov/pubmed/37923834
http://dx.doi.org/10.1038/s41598-023-46314-6

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10624827/
https://www.ncbi.nlm.nih.gov/pubmed/37923834
http://dx.doi.org/10.1038/s41598-023-46314-6

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