Cargando…

A duplex droplet digital PCR assay for absolute quantification and characterization of long self-amplifying RNA

Self-amplifying messenger ribonucleic acid (saRNA) provides extended expression of genes of interest by encoding an alphavirus-derived RNA replicase and thus is 2–3 times larger than conventional messenger RNA. However, quality assessment of long RNA transcripts is challenging using standard techniq...

Descripción completa

Detalles Bibliográficos
Autores principales:	Casmil, Irafasha C., Huang, Cynthia, Blakney, Anna K.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group UK 2023
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10624827/ https://www.ncbi.nlm.nih.gov/pubmed/37923834 http://dx.doi.org/10.1038/s41598-023-46314-6

Ejemplares similares

Development of a duplex droplet digital PCR assay for absolute quantitative detection of "Candidatus Liberibacter asiaticus"
por: Selvaraj, Vijayanandraj, et al.
Publicado: (2018)

Absolute quantification by droplet digital PCR versus analog real-time PCR
por: Hindson, Christopher M, et al.
Publicado: (2013)

Detection and quantification of beef and pork materials in meat products by duplex droplet digital PCR
por: Cai, Yicun, et al.
Publicado: (2017)

Development and Evaluation of a Single Dye Duplex Droplet Digital PCR Assay for the Rapid Detection and Quantification of Mycobacterium tuberculosis
por: Nyaruaba, Raphael, et al.
Publicado: (2020)

Droplet volume variability as a critical factor for accuracy of absolute quantification using droplet digital PCR
por: Košir, Alexandra Bogožalec, et al.
Publicado: (2017)

Droplet Digital Enzyme-Linked Oligonucleotide Hybridization Assay for Absolute RNA Quantification
por: Guan, Weihua, et al.
Publicado: (2015)

Droplet Digital PCR for absolute quantification and determination of proviral load of HTLV-1 and 2
por: Hedberg, Sara Thulin, et al.
Publicado: (2015)

Evaluation of Digital PCR for Absolute RNA Quantification
por: Sanders, Rebecca, et al.
Publicado: (2013)

Quantitative Evaluation of CFTR Gene Expression: A Comparison between Relative Quantification by Real-Time PCR and Absolute Quantification by Droplet Digital PCR
por: Bruno, Sabina Maria, et al.
Publicado: (2023)

Evaluation of Droplet Digital Polymerase Chain Reaction (ddPCR) for the Absolute Quantification of Aspergillus species in the Human Airway
por: Poh, Tuang Yeow, et al.
Publicado: (2020)

Absolute Quantification of Viable but Nonculturable Vibrio cholerae Using Droplet Digital PCR with Oil-Enveloped Bacterial Cells
por: Zhao, Shuo, et al.
Publicado: (2022)

Absolute quantification of Mycoplasma pneumoniae in infected patients by droplet digital PCR to track disease severity and treatment efficacy
por: Zhao, Hanqing, et al.
Publicado: (2023)

Quantification of HEV RNA by Droplet Digital PCR
por: Nicot, Florence, et al.
Publicado: (2016)

Droplet Digital PCR for Estimating Absolute Abundances of Widespread Pelagibacter Viruses
por: Martinez-Hernandez, Francisco, et al.
Publicado: (2019)

Validation of an ultrasensitive digital droplet PCR assay for HIV-2 plasma RNA quantification
por: Ruelle, Jean, et al.
Publicado: (2014)

A Duplex-Droplet Digital PCR Assay for Simultaneous Quantitative Detection of Monilinia fructicola and Monilinia laxa on Stone Fruits
por: Raguseo, Celeste, et al.
Publicado: (2021)

Absolute quantification of viable Vibrio cholerae in seawater samples using multiplex droplet digital PCR combined with propidium monoazide
por: Yang, Jinsong, et al.
Publicado: (2023)

Droplet digital PCR (ddPCR) for the detection and quantification of Ureaplasma spp.
por: Huang, Yanfang, et al.
Publicado: (2021)

A droplet digital PCR assay for detection and quantification of Verticillium nonalfalfae and V. albo-atrum
por: Wang, Di, et al.
Publicado: (2023)

Respiratory Adenovirus Quantification with a Droplet Digital Polymerase Chain Reaction (ddPCR) Assay
por: Abdullah, Omar, et al.
Publicado: (2023)

Digital Droplet PCR for the Absolute Quantification of Exon Skipping Induced by Antisense Oligonucleotides in (Pre-)Clinical Development for Duchenne Muscular Dystrophy
por: Verheul, Ruurd C., et al.
Publicado: (2016)

Fast Prototyping Microfluidics: Integrating Droplet Digital Lamp for Absolute Quantification of Cancer Biomarkers
por: Oliveira, Beatriz, et al.
Publicado: (2020)

Impact of variance components on reliability of absolute quantification using digital PCR
por: Jacobs, Bart KM, et al.
Publicado: (2014)

Diagnostic utility of droplet digital PCR for HIV reservoir quantification
por: Trypsteen, Wim, et al.
Publicado: (2016)

Droplet digital PCR assay provides intrahepatic HBV cccDNA quantification tool for clinical application
por: Hayashi, Sanae, et al.
Publicado: (2022)

High-Throughput Droplet Digital PCR System for Absolute Quantitation of DNA Copy Number
por: Hindson, Benjamin J., et al.
Publicado: (2011)

A Novel Pretreatment-Free Duplex Chamber Digital PCR Detection System for the Absolute Quantitation of GMO Samples
por: Zhu, Pengyu, et al.
Publicado: (2016)

Development, Optimization, and Evaluation of a Duplex Droplet Digital PCR Assay To Quantify the T-nos/hmg Copy Number Ratio in Genetically Modified Maize
por: Dalmira, Félix-Urquídez, et al.
Publicado: (2015)

Absolute quantification of SARS-CoV-2 with Clarity Plus™ digital PCR
por: Tan, Shawn Yi Han, et al.
Publicado: (2022)

Droplet digital PCR for quantification of ITGA6 in a stool mRNA assay for the detection of colorectal cancers
por: Herring, Elizabeth, et al.
Publicado: (2017)

Maximizing viral detection with SIV droplet digital PCR (ddPCR) assays
por: Long, Samuel, et al.
Publicado: (2020)

Quantification of Plasmid Copy Number with Single Colour Droplet Digital PCR
por: Plotka, Magdalena, et al.
Publicado: (2017)

Droplet digital PCR for the quantification of Alu methylation status in hematological malignancies
por: Orsini, Paola, et al.
Publicado: (2018)

Four human Plasmodium species quantification using droplet digital PCR
por: Srisutham, Suttipat, et al.
Publicado: (2017)

Quantification of circulating microRNAs by droplet digital PCR for cancer detection
por: Cirillo, Priscila D. R., et al.
Publicado: (2020)

Digital-Droplet PCR for Quantification of CD19-Directed CAR T-Cells
por: Mika, Thomas, et al.
Publicado: (2020)

HIV reservoir quantification by five-target multiplex droplet digital PCR
por: Levy, Claire N., et al.
Publicado: (2021)

Clinical Application of Droplet Digital PCR for Hepatitis Delta Virus Quantification
por: Olivero, Antonella, et al.
Publicado: (2022)

Simultaneous Giant Virus and Virophage Quantification Using Droplet Digital PCR
por: del Arco, Ana, et al.
Publicado: (2022)

Development of the Droplet Digital PCR Method for the Detection and Quantification of Erwinia pyrifoliae
por: He, Lin, et al.
Publicado: (2023)

Cannot write session to /tmp/vufind_sessions/sess_co4sbf1cti7qtdticflhdcj5tb