A framework to validate fluorescently labeled DNA-binding proteins for single-molecule experiments

Due to the enhanced labeling capability of maleimide-based fluorescent probes, lysine-cysteine-lysine (KCK) tags are frequently added to proteins for visualization. In this study, we employed an in vitro single-molecule DNA flow-stretching assay as a sensitive way to assess the impact of the KCK tag...

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Detalles Bibliográficos
Autores principales: Molina, Miranda, Way, Lindsey E., Ren, Zhongqing, Liao, Qin, Guerra, Bianca, Shields, Brandon, Wang, Xindan, Kim, HyeongJun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Resource
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10626211/
https://www.ncbi.nlm.nih.gov/pubmed/37832544
http://dx.doi.org/10.1016/j.crmeth.2023.100614

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10626211/
https://www.ncbi.nlm.nih.gov/pubmed/37832544
http://dx.doi.org/10.1016/j.crmeth.2023.100614

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