Cargando…

A framework to validate fluorescently labeled DNA-binding proteins for single-molecule experiments

Due to the enhanced labeling capability of maleimide-based fluorescent probes, lysine-cysteine-lysine (KCK) tags are frequently added to proteins for visualization. In this study, we employed an in vitro single-molecule DNA flow-stretching assay as a sensitive way to assess the impact of the KCK tag...

Descripción completa

Detalles Bibliográficos
Autores principales:	Molina, Miranda, Way, Lindsey E., Ren, Zhongqing, Liao, Qin, Guerra, Bianca, Shields, Brandon, Wang, Xindan, Kim, HyeongJun
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2023
Materias:	Resource
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10626211/ https://www.ncbi.nlm.nih.gov/pubmed/37832544 http://dx.doi.org/10.1016/j.crmeth.2023.100614

Ejemplares similares

Using DNA flow-stretching assay as a tool to validate the tagging of DNA-binding proteins for single-molecule experiments
por: Molina, Miranda, et al.
Publicado: (2023)

Triple S-Phase Labeling of Dividing Stem Cells
por: Podgorny, Oleg, et al.
Publicado: (2018)

A solid-state nanopore-based single-molecule approach for label-free characterization of plant polysaccharides
por: Cai, Yao, et al.
Publicado: (2020)

Fluorescent Gene Tagging of Transcriptionally Silent Genes in hiPSCs
por: Roberts, Brock, et al.
Publicado: (2019)

Dynamics of Translation of Single mRNA Molecules In Vivo
por: Yan, Xiaowei, et al.
Publicado: (2016)

The Cardiomyocyte RNA-Binding Proteome: Links to Intermediary Metabolism and Heart Disease
por: Liao, Yalin, et al.
Publicado: (2016)

Genome-wide bimolecular fluorescence complementation analysis of SUMO interactome in yeast
por: Sung, Min-Kyung, et al.
Publicado: (2013)

Quantitative analysis of human centrosome architecture by targeted proteomics and fluorescence imaging
por: Bauer, Manuel, et al.
Publicado: (2016)

Automatic identification of small molecules that promote cell conversion and reprogramming
por: Napolitano, Francesco, et al.
Publicado: (2021)

An Endogenously Tagged Fluorescent Fusion Protein Library in Mouse Embryonic Stem Cells
por: Harikumar, Arigela, et al.
Publicado: (2017)

Amine Landscaping to Maximize Protein-Dye Fluorescence and Ultrastable Protein-Ligand Interaction
por: Jacobsen, Michael T., et al.
Publicado: (2017)

Single-molecule identification of the target RNAs of different RNA binding proteins simultaneously in cells
por: Flamand, Mathieu N., et al.
Publicado: (2022)

Direct fluorescent labeling of NF186 and Na(V)1.6 in living primary neurons using bioorthogonal click chemistry
por: Stajković, Nevena, et al.
Publicado: (2023)

LIVECell—A large-scale dataset for label-free live cell segmentation
por: Edlund, Christoffer, et al.
Publicado: (2021)

Small-Molecule-Driven Hepatocyte Differentiation of Human Pluripotent Stem Cells
por: Siller, Richard, et al.
Publicado: (2015)

High-Throughput Assay and Discovery of Small Molecules that Interrupt Malaria Transmission
por: Plouffe, David M., et al.
Publicado: (2016)

Decreased synthesis of ribosomal proteins in tauopathy revealed by non‐canonical amino acid labelling
por: Evans, Harrison Tudor, et al.
Publicado: (2019)

A comparative transcriptional landscape of maize and sorghum obtained by single-molecule sequencing
por: Wang, Bo, et al.
Publicado: (2018)

Generation of pancreatic progenitors from human pluripotent stem cells by small molecules
por: Jiang, Yuqian, et al.
Publicado: (2021)

Evolutionary Interrogation of Human Biology in Well-Annotated Genomic Framework of Rhesus Macaque
por: Zhang, Shi-Jian, et al.
Publicado: (2014)

Deep Mutational Scanning of SARS-CoV-2 Receptor Binding Domain Reveals Constraints on Folding and ACE2 Binding
por: Starr, Tyler N., et al.
Publicado: (2020)

Building a genomic framework for prospective MRSA surveillance in the United Kingdom and the Republic of Ireland
por: Reuter, Sandra, et al.
Publicado: (2016)

A curated census of autophagy-modulating proteins and small molecules: Candidate targets for cancer therapy
por: Lorenzi, Philip L, et al.
Publicado: (2014)

Comprehensive Identification of RNA-Binding Domains in Human Cells
por: Castello, Alfredo, et al.
Publicado: (2016)

Deep mutational learning predicts ACE2 binding and antibody escape to combinatorial mutations in the SARS-CoV-2 receptor-binding domain
por: Taft, Joseph M., et al.
Publicado: (2022)

A Scaled Framework for CRISPR Editing of Human Pluripotent Stem Cells to Study Psychiatric Disease
por: Hazelbaker, Dane Z., et al.
Publicado: (2017)

Computational Framework for High-Quality Production and Large-Scale Evolutionary Analysis of Metagenome Assembled Genomes
por: Murovec, Boštjan, et al.
Publicado: (2020)

Pan‐genome and multi‐parental framework for high‐resolution trait dissection in melon (Cucumis melo)
por: Oren, Elad, et al.
Publicado: (2022)

Isolation of Progenitors that Exhibit Myogenic/Osteogenic Bipotency In Vitro by Fluorescence-Activated Cell Sorting from Human Fetal Muscle
por: Castiglioni, Alessandra, et al.
Publicado: (2014)

Regulation of alternative splicing in Drosophila by 56 RNA binding proteins
por: Brooks, Angela N., et al.
Publicado: (2015)

A patient-centric modeling framework captures recovery from SARS-CoV-2 infection
por: Ruffieux, Hélène, et al.
Publicado: (2023)

CodABC: A Computational Framework to Coestimate Recombination, Substitution, and Molecular Adaptation Rates by Approximate Bayesian Computation
por: Arenas, Miguel, et al.
Publicado: (2015)

TDTHub, a web server tool for the analysis of transcription factor binding sites in plants
por: Grau, Joaquín, et al.
Publicado: (2022)

Insight into small molecule binding to the neonatal Fc receptor by X-ray crystallography and 100 kHz magic-angle-spinning NMR
por: Stöppler, Daniel, et al.
Publicado: (2018)

SARS-COV-2 spike binding to ACE2 in living cells monitored by TR-FRET
por: Cecon, Erika, et al.
Publicado: (2022)

SALL1 enforces microglia-specific DNA binding and function of SMADs to establish microglia identity
por: Fixsen, Bethany R., et al.
Publicado: (2023)

Characterization and complexity of transcriptome in Gymnocypris przewalskii using single-molecule long-read sequencing and RNA-seq
por: Li, Xindan, et al.
Publicado: (2021)

Subtelomeric CTCF and cohesin binding site organization using improved subtelomere assemblies and a novel annotation pipeline
por: Stong, Nicholas, et al.
Publicado: (2014)

Dynamic Analysis of Gene Expression and Genome-wide Transcription Factor Binding during Lineage Specification of Multipotent Progenitors
por: May, Gillian, et al.
Publicado: (2013)

Derivation and FACS-Mediated Purification of PAX3+/PAX7+ Skeletal Muscle Precursors from Human Pluripotent Stem Cells
por: Borchin, Bianca, et al.
Publicado: (2013)

Cannot write session to /tmp/vufind_sessions/sess_fktg5mhc4pj0mldm0jpq0g8v2l