Rapid and sensitive one-tube detection of mpox virus using RPA-coupled CRISPR-Cas12 assay

Mpox is caused by a zoonotic virus belonging to the Orthopoxvirus genus and the Poxviridae family. In this study, we develop a recombinase polymerase amplification (RPA)-coupled CRISPR-Cas12a detection assay for the mpox virus. We design and test a series of CRISPR-derived RNAs(crRNAs) targeting the...

Descripción completa

Detalles Bibliográficos
Autores principales: Zhao, Fei, Hu, Yamei, Fan, Zhangling, Huang, Baoying, Wei, Liang, Xie, Yu, Huang, Yu, Mei, Shan, Wang, Liming, Wang, Lingwa, Ai, Bin, Fang, Jugao, Liang, Chen, Xu, Fengwen, Tan, Wenjie, Guo, Fei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10626268/
https://www.ncbi.nlm.nih.gov/pubmed/37848032
http://dx.doi.org/10.1016/j.crmeth.2023.100620

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10626268/
https://www.ncbi.nlm.nih.gov/pubmed/37848032
http://dx.doi.org/10.1016/j.crmeth.2023.100620

Ejemplares similares