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Rapid and sensitive one-tube detection of mpox virus using RPA-coupled CRISPR-Cas12 assay

Mpox is caused by a zoonotic virus belonging to the Orthopoxvirus genus and the Poxviridae family. In this study, we develop a recombinase polymerase amplification (RPA)-coupled CRISPR-Cas12a detection assay for the mpox virus. We design and test a series of CRISPR-derived RNAs(crRNAs) targeting the...

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Detalles Bibliográficos
Autores principales:	Zhao, Fei, Hu, Yamei, Fan, Zhangling, Huang, Baoying, Wei, Liang, Xie, Yu, Huang, Yu, Mei, Shan, Wang, Liming, Wang, Lingwa, Ai, Bin, Fang, Jugao, Liang, Chen, Xu, Fengwen, Tan, Wenjie, Guo, Fei
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2023
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10626268/ https://www.ncbi.nlm.nih.gov/pubmed/37848032 http://dx.doi.org/10.1016/j.crmeth.2023.100620

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